Ferritin Nanocage Conjugated Hybrid Hydrogel for Tissue Engineering and Drug Delivery Applications.
Ferritin Nanocage Conjugated Hybrid Hydrogel for Tissue Engineering and Drug Delivery Applications.
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DOI：
10.1021/acsbiomaterials.9b01482
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发表时间：
2020-01-13
影响因子：
5.8
通讯作者：
Shin SR
中科院分区：
文献类型：
--
作者：
Samanipour R;Wang T;Werb M;Hassannezhad H;Rangel JML;Hoorfar M;Hasan A;Lee CK;Shin SR
水凝胶由于其与天然细胞外基质的结构相似性，最近在各种药物递送和组织工程应用中具有吸引力。尽管在水凝胶的应用方面取得了巨大的进步，但差的机械性能和缺乏对药物和生物分子释放的控制是广泛临床应用的主要障碍。为了克服这些挑战，我们开发了物理和共价结合的纳米笼负载的水凝胶之间的表面的纳米笼和明胶甲基丙烯酰基（GelMA）水凝胶基质。铁蛋白及其空核等效物脱铁铁蛋白被用作纳米笼，其可以通过物理键合容易地并入GelMA水凝胶中。为了制备共价缀合的纳米笼负载的GelMA水凝胶，铁蛋白和脱铁铁蛋白进行化学改性以分别呈现甲基丙烯酰基，铁蛋白甲基丙烯酰基（FerMA）和脱铁铁蛋白甲基丙烯酰基（ApoMA）。共价结合的FerMA-和ApoMA-GelMA水凝胶提供了更好的能力，以调整机械性能相比，通过直接分散的铁蛋白和脱铁铁蛋白到GelMA水凝胶与物理键合，而不影响其孔隙率或细胞生长。此外，通过对异硫氰酸荧光素（FITC）包封的脱铁铁蛋白和ApoMA掺入的GelMA水凝胶通过pH刺激进行累积释放测试，证实了纳米笼释放小化合物的能力。因此，纳米笼结合的水凝胶已经成为药物递送和组织工程应用的优异材料。
Hydrogels have recently been attractive in various drug delivery and tissue engineering applications because of their structural similarities to the natural extracellular matrix. Despite enormous advances in the application of hydrogels, poor mechanical properties and lack of control for the release of drugs and biomolecules act as major barriers for widespread clinical applications. To overcome these challenges, we developed both physically and covalently conjugated nanocage-laden hydrogels between the surface of the nanocage and a gelatin methacryloyl (GelMA) hydrogel matrix. Ferritin and its empty-core equivalent apoferritin were used as nanocages that could be easily incorporated into a GelMA hydrogel via physical bonding. To fabricate covalently conjugated nanocage-laden GelMA hydrogels, ferritin and apoferritin were chemically modified to present the methacryloyl groups, ferritin methacryloyl (FerMA) and apoferritin methacryloyl (ApoMA), respectively. The covalently conjugated FerMA- and ApoMA-GelMA hydrogels offered a better ability to tune mechanical properties compared with those prepared by direct dispersion of ferritin and apoferritin into GelMA hydrogels with physical bonding, without affecting their porosity or cell growth. Furthermore, the ability of the nanocage to release small chemical compounds was confirmed by performing a cumulative release test on fluorescein isothiocyanate (FITC) encapsulated apoferritin and ApoMA incorporated GelMA hydrogels by pH stimulus. Thus, the nanocage incorporated hydrogels have emerged as excellent materials for drug delivery and tissue engineering applications.
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影响因子：
9.5
作者：
Shanmuganathan, Kadhiravan;Capadona, Jeffrey R.;Rowan, Stuart J.;Weder, Christoph
通讯作者：
Weder, Christoph
影响因子：
29.4
作者：
Shin, Min Kyoon;Spinks, Geoffrey M.;Kim, Seon Jeong
通讯作者：
Kim, Seon Jeong
影响因子：
17.1
作者：
Shin, Su Ryon;Jung, Sung Mi;Zalabany, Momen;Kim, Keekyoung;Zorlutuna, Pinar;Kim, Sang Bok;Nikkhah, Mehdi;Khabiry, Masoud;Azize, Mohamed;Kong, Jing;Wan, Kai-tak;Palacios, Tomas;Dokmeci, Mehmet R.;Bae, Hojae;Tang, Xiaowu (Shirley);Khademhosseini, Ali
通讯作者：
Khademhosseini, Ali
影响因子：
17.1
作者：
Shin SR;Bae H;Cha JM;Mun JY;Chen YC;Tekin H;Shin H;Zarabi S;Dokmeci MR;Tang S;Khademhosseini A
通讯作者：
Khademhosseini A
影响因子：
4
作者：
Shin, Min Kyoon;Kim, Sun I.;Baughman, Ray H.
通讯作者：
Baughman, Ray H.