Carbon nanotube reinforced hybrid microgels as scaffold materials for cell encapsulation.
Carbon nanotube reinforced hybrid microgels as scaffold materials for cell encapsulation.
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DOI：
10.1021/nn203711s
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发表时间：
2012-01-24
期刊：
影响因子：
17.1
通讯作者：
Khademhosseini A
中科院分区：
文献类型：
--
作者：
Shin SR;Bae H;Cha JM;Mun JY;Chen YC;Tekin H;Shin H;Zarabi S;Dokmeci MR;Tang S;Khademhosseini A
模拟生物细胞外基质（ECM）的水凝胶可以为细胞提供机械支持和信号线索，以调节其行为。然而，尽管水凝胶能够产生可以调节细胞行为的人工ECM，但它们通常缺乏许多组织构建体所需的机械强度。在这里，我们提出了加强CNT-明胶甲基丙烯酸酯（GelMA）混合作为生物相容性，细胞响应性水凝胶平台，用于创建细胞负载的三维（3D）结构。CNT的加入成功地增强了GelMA水凝胶，而不降低其孔隙率或抑制细胞生长。CNT-GelMA杂化物也是可图案化的，允许在没有苛刻工艺的情况下容易地制造微尺度结构。NIH-3 T3细胞和人间充质干细胞（hMSC）在CNT-GelMA混合微凝胶中包封后容易扩散和增殖。通过控制并入GelMA水凝胶系统中的CNT的量，我们证明了可以调整混合材料的机械性能，使其适合于各种组织工程应用。此外，由于引入CNT的GelMA的高图案保真度和分辨率，其可用于体外细胞研究或制造复杂的3D仿生组织样结构。
Hydrogels that mimic biological extracellular matrix (ECM) can provide cells with mechanical support and signaling cues to regulate their behavior. However, despite the ability of hydrogels to generate artificial ECM that can modulate cellular behavior, they often lack the mechanical strength needed for many tissue constructs. Here, we present reinforced CNT-gelatin methacrylate (GelMA) hybrid as a biocompatible, cell-responsive hydrogel platform for creating cell-laden three dimensional (3D) constructs. The addition of CNTs successfully reinforced GelMA hydrogels without decreasing their porosity or inhibiting cell growth. The CNT-GelMA hybrids were also photopatternable allowing for easy fabrication of microscale structures without harsh processes. NIH-3T3 cells and human mesenchymal stem cells (hMSCs) readily spread and proliferated after encapsulation in CNT-GelMA hybrid microgels. By controlling the amount of CNTs incorporated into the GelMA hydrogel system, we demonstrated that the mechanical properties of the hybrid material can be tuned making it suitable for various tissue engineering applications. Furthermore, due to the high pattern fidelity and resolution of CNT incorporated GelMA, it can be used for in vitro cell studies or fabricating complex 3D biomimetic tissue-like structures.
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影响因子：
14
作者：
Aubin H;Nichol JW;Hutson CB;Bae H;Sieminski AL;Cropek DM;Akhyari P;Khademhosseini A
通讯作者：
Khademhosseini A
影响因子：
3.7
作者：
Mu, Qingxin;Liu, Wei;Yan, Bing
通讯作者：
Yan, Bing
影响因子：
14
作者：
Kumachev, Alexander;Greener, Jesse;Kumacheva, Eugenia
通讯作者：
Kumacheva, Eugenia
影响因子：
9
作者：
Hwang CM;Sant S;Masaeli M;Kachouie NN;Zamanian B;Lee SH;Khademhosseini A
通讯作者：
Khademhosseini A
影响因子：
3.7
作者：
Galano, Annia
通讯作者：
Galano, Annia