Purinergic receptor signaling regulates N-cadherin expression in primary astrocyte cultures.
Purinergic receptor signaling regulates N-cadherin expression in primary astrocyte cultures.
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嘌呤能受体信号传导调节原代星形胶质细胞培养物中 N-钙粘蛋白的表达。
DOI：
10.1111/j.1471-4159.2008.05214.x
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发表时间：
2008
影响因子：
4.7
通讯作者：
Neary,JosephT
中科院分区：
文献类型：
--
作者：
Tran,MinhD;Wanner,InaB;Neary,JosephT
Extracellular ATP exerts both short‐term and long‐term effects in the CNS by stimulating cell‐surface purinergic receptors. Here we have examined the effect of purinergic receptor activation on N‐cadherin expression, a calcium‐dependent cell adhesion molecule involved in many processes, including glia‐glia and axon‐glia interactions. When primary cultures of rat cortical astrocytes were treated with ATP, N‐cadherin protein expression increased in a time‐ and concentration‐dependent manner. In addition, ATP treatment caused an increase in N‐cadherin immunoreactivity in both the cytoplasm and on the cell surface membrane. Interestingly, experiments with cycloheximide revealed that relocalization of N‐cadherin to the cell surface membrane were independent of protein synthesis. The ATP‐induced increase in N‐cadherin protein expression was blocked by reactive blue 2 and 8‐(p‐sulfophenyl)‐theophylline, suggesting involvement of both P2 and P1 purinergic receptors, respectively. In addition, N‐cadherin expression was partially blocked when signaling from purinergic receptors to extracellular signal regulated protein kinase or Akt was inhibited by 1,4‐diamino‐2,3‐dicyano‐1,4‐bis(2‐aminophenylthio)butadiene or wortmannin, respectively. By using anin vitromodel of traumatic CNS injury, we found that N‐cadherin expression was increased when astrocytes were subjected to rapid and reversible mechanical strain. The findings presented here demonstrate a role for extracellular ATP, purinergic receptors and protein kinase signaling in regulating N‐cadherin expression and suggest a role for this mechanism in cell‐cell interactions.
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影响因子：
5.4
作者：
T. Ooka;G. Lenoir;J. Daillie
通讯作者：
J. Daillie
两种相关的 Epstein-Barr 病毒编码膜蛋白的表征，这两种蛋白在伯基特淋巴瘤和体外转化细胞系中差异表达。
DOI：
10.1073/pnas.83.15.5703
发表时间：
1986
影响因子：
11.1
作者：
S. Modrow;H. Wolf
通讯作者：
H. Wolf
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DOI：
--
发表时间：
1979
影响因子：
11.1
作者：
G. Klein
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G. Klein
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影响因子：
5.4
作者：
H. Delius;G. Bornkamm
通讯作者：
G. Bornkamm
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影响因子：
6.4
作者：
I. Ernberg;B. Kallin;J. Dillner;K. Falk;B. Ehlin‐Henriksson;M. Hammarskjöld;G. Klein
通讯作者：
G. Klein