Development of near-infrared mitochondrial polarity fluorescent probe for evaluating mitophagy in mice heart and potential cancer diagnosis
Development of near-infrared mitochondrial polarity fluorescent probe for evaluating mitophagy in mice heart and potential cancer diagnosis
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开发近红外线粒体极性荧光探针，用于评估小鼠心脏的线粒体自噬和潜在的癌症诊断
DOI：
10.1016/j.cej.2022.135397
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发表时间：
2022-02
影响因子：
15.1
通讯作者：
Caixia Yin
中科院分区：
文献类型：
--
作者：
Tao Zhang;Fangjun Huo;Weijie Zhang;Fangqin Cheng;Caixia Yin
线粒体自噬是选择性去除多余或受损线粒体，并调节细胞内线粒体数量以维持正常线粒体功能的过程。据报道，线粒体自噬可以引起细胞内微环境的显着变化，例如影响细胞极性。因此，检测细胞内微环境极性的变化是监测自噬过程的有效手段。在此，我们合成了一种极性敏感荧光探针NIR-BT-P，具有强大的ICT机制和900 nm近红外区域。该探针由鎓盐作为缺电子母体荧光团和三苯胺作为强给电子基团构建。研究表明，NIR-BT-P的荧光强度随着环境极性的降低而增加，并伴随着最大发射波长的红移（812 nm-853 nm）。此外，NIR-BT-P不受粘度、pH或常见活性物质的干扰。值得注意的是，探针NIR-BT-P可以实时监测饥饿和药物诱导的线粒体自噬，并成功区分正常细胞和癌细胞。更重要的是，通过对饥饿小鼠组心脏的心肌细胞进行可视化，发现心脏饥饿期间发生了线粒体自噬。因此，我们预计 NIR-BT-P 不仅可以深入了解线粒体自噬与心脏病之间的关系，而且还可能成为癌症临床诊断的潜在工具。
Mitophagy is a process that selectively removes excess or damaged mitochondria, and regulates the number of mitochondria in the cell to maintain the normal mitochondrial function. According to reports, mitophagy can cause significant changes to the intracellular microenvironment, such as affecting cell polarity. Therefore, detecting changes in polarity in the intracellular microenvironment is an effective means to monitor the autophagy process. Herein, we synthesized a polarity-sensitive fluorescent probeNIR-BT-Pwith a strong ICT mechanism and 900 nm near infrared region. The probe was constructed by an onium salt as the electron-deficient parent fluorophore and triphenylamine as a strong electron donating group. Studies indicated that the fluorescence intensity ofNIR-BT-Pincreases with decreasing environmental polarity, accompanied by a redshift (812 nm-853 nm) of the maximum emission wavelength. In addition,NIR-BT-Pdid not interfere by viscosity, pH, or common active substances. Notably, the probeNIR-BT-Pcan monitor starvation and drug-induced mitophagy in real time, and successfully distinguished normal cells from cancer cells. More importantly, by visualizing the cardiomyocytes of the heart of the starvation mice group, it was found that mitophagy occurred during heart starvation. Consequently, we anticipate thatNIR-BT-Pcould not only provide insight into the relationship between mitophagy and heart disease, but may also emerge as a potential tool for the clinical diagnosis of cancer.
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