Ectopic Expression of Sugarcane ScAMT1.1 Has the Potential to Improve Ammonium Assimilation and Grain Yield in Transgenic Rice under Low Nitrogen Stress.
Ectopic Expression of Sugarcane ScAMT1.1 Has the Potential to Improve Ammonium Assimilation and Grain Yield in Transgenic Rice under Low Nitrogen Stress.
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甘蔗ScAMT1.1的异位表达有可能提高低氮胁迫下转基因水稻的铵同化和籽粒产量
DOI：
10.3390/ijms24021595
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发表时间：
2023-01-13
影响因子：
5.6
通讯作者：
Xu, Liping
中科院分区：
文献类型：
--
作者：
Gao, Shiwu;Yang, Yingying;Guo, Jinlong;Zhang, Xu;Feng, Minxie;Su, Yachun;Que, Youxiong;Xu, Liping
在我国，甘蔗种植区氮肥施用过量，而甘蔗氮肥利用率较低。挖掘和鉴定甘蔗响应低氮胁迫的关键基因，可以为开发高NUE甘蔗品种提供有用的基因元件和理论依据。在我们的研究中，RNA-Seq结合qRT-PCR分析表明，ScAMT1.1基因对低氮胁迫有积极响应，导致甘蔗品种ROC22具有更强的低氮耐受性和高NUE能力。然后，从甘蔗中克隆了ScAMT1.1。 ScAMT1.1基因的cDNA全长1868bp，包含1491bp的开放阅读框（ORF），编码496个氨基酸。 ScAMT1.1属于AMT超家族，与水稻AMT1.1有91.57%的同源性。此外，它在水稻（O. sativa）中稳定过度表达。低氮处理下，ScAMT1.1过表达转基因水稻的株高和鲜重分别比野生型高36.48%和51.55%。转基因植株中铵同化关键酶GS和GDH的活性以及铵同化关键基因GS1.1、GS1.2、GDH、Fd-GOGAT、NADH-GOGAT2的表达量均显着高于野生型。盆栽试验中转基因水稻的粒数和单株籽粒产量分别比野生型提高了6.44%和9.52%。综上所述，甘蔗ScAMT1.1基因具有提高低氮肥条件下铵同化能力和转基因水稻产量的潜力。该研究为改良高NUE甘蔗品种提供了重要的功能基因。
In China, nitrogen (N) fertilizer is excessively used in sugarcane planting areas, while the nitrogen use efficiency (NUE) of sugarcane is relatively low. Mining and identifying the key genes in response to low N stress in sugarcane can provide useful gene elements and a theoretical basis for developing sugarcane varieties with high NUE. In our study, RNA-Seq combined with qRT-PCR analysis revealed that the ScAMT1.1 gene responded positively to low N stress, resulting in the stronger low N tolerance and high NUE ability of sugarcane cultivar ROC22. Then, ScAMT1.1 was cloned from sugarcane. The full-length cDNA of the ScAMT1.1 gene is 1868 bp, containing a 1491 bp open reading frame (ORF), and encoding 496 amino acids. ScAMT1.1 belongs to the AMT superfamily and shares 91.57% homologies with AMT1.1 from Oryza sativa. Furthermore, it was stably overexpressed in rice (O. sativa). Under low N treatment, the plant height and the fresh weight of the ScAMT1.1-overexpressed transgenic rice were 36.48% and 51.55% higher than that of the wild-type, respectively. Both the activity of ammonium assimilation key enzymes GS and GDH, and the expression level of ammonium assimilation key genes, including GS1.1, GS1.2, GDH, Fd-GOGAT, and NADH-GOGAT2 in the transgenic plants, were significantly higher than that of the wild-type. The grain number and grain yield per plant in the transgenic rice were 6.44% and 9.52% higher than that of the wild-type in the pot experiments, respectively. Taken together, the sugarcane ScAMT1.1 gene has the potential to improve ammonium assimilation ability and the yield of transgenic rice under low N fertilizer conditions. This study provided an important functional gene for improving sugarcane varieties with high NUE.
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影响因子：
11.4
作者：
MARINI, AM;VISSERS, S;ANDRE, B
通讯作者：
ANDRE, B
影响因子：
7.4
作者：
Kaiser, BN;Rawat, SR;Glass, ADM
通讯作者：
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2.1
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通讯作者：
Loit, Evelin
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4.9
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通讯作者：
Yamaguchi, J
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作者：
Kronzucker, HJ;Siddiqi, MY;Glass, ADM
通讯作者：
Glass, ADM