DR1440 is a potential iron efflux protein involved in maintenance of iron homeostasis and resistance of Deinococcus radiodurans to oxidative stress.
DR1440 is a potential iron efflux protein involved in maintenance of iron homeostasis and resistance of Deinococcus radiodurans to oxidative stress.
复制标题
DR1440 是一种潜在的铁流出蛋白，参与铁稳态的维持和耐辐射球菌对氧化应激的抵抗
DOI：
10.1371/journal.pone.0202287
复制
发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
Hua Y
中科院分区：
文献类型：
--
作者：
Dai S;Jin Y;Li T;Weng Y;Xu X;Zhang G;Li J;Pang R;Tian B;Hua Y
细菌获取铁的研究很好，但细菌铁输出的了解较少。在此，我们确定dr1440与P型ATP酶基序作为一个潜在的出口商的铁从耐辐射异常球菌，一种细菌，其极端耐辐射和氧化剂。通过荧光标记分析证明DR1440位于细胞膜上。dr1440突变导致铁离子在细胞内积累，在铁离子或过氧化氢胁迫下，野生型菌株dr1440的表达水平显著上调，提示DR1440可能是一种潜在的铁外排蛋白。与野生型菌株相比，dr1440突变体对铁离子和氧化应激（包括过氧化氢、次氯酸和γ射线照射）表现出更高的敏感性。突变株中高含量的铁导致严重的蛋白质羰基化，表明DR1440可能有助于细胞内蛋白质保护免受亚铁离子介导的Fenton反应产生的活性氧（ROS）。S297 A和C299 A突变导致细胞内铁积累，表明S297和C299可能是DR1440的重要功能残基。因此，DR1440可能是一种与铁稳态和抗氧化应激有关的铁外排蛋白。耐辐射的
Iron acquisition by bacteria is well studied, but iron export from bacteria is less understood. Herein, we identified dr1440 with a P-type ATPase motif as a potential exporter of iron from Deinococcus radiodurans, a bacterium known for its extreme resistance to radiation and oxidants. The DR1440 was located in cell membrane as demonstrated by fluorescence labelling analysis. Mutation of dr1440 resulted in cellular accumulation of iron ions, and expression level of dr1440 was up-regulated significantly under iron ion or hydrogen peroxide stress in the wild-type strain, implicating DR1440 as a potential iron efflux protein. The dr1440 mutant displayed higher sensitivity to iron ions and oxidative stresses including hydrogen peroxide, hypochlorous acid, and gamma-ray irradiation compared with the wild-type strain. The high amount of iron in the mutant strain resulted in severe protein carbonylation, suggesting that DR1440 might contribute to intracellular protein protection against reactive oxygen species (ROS) generated from ferrous ion-mediated Fenton-reaction. Mutations of S297A and C299A led to intracellular accumulation of iron, indicating that S297 and C299 might be important functional residues of DR1440. Thus, DR1440 is a potential iron efflux protein involved in iron homeostasis and oxidative stress-resistance of D. radiodurans.
登录
查看更多内容
影响因子：
4.8
作者：
Fan, B;Rosen, BP
通讯作者：
Rosen, BP
DOI：
10.1073/pnas.78.2.1001
发表时间：
1981-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
REPINE, JE;PFENNINGER, OW;PETTIJOHN, DE
通讯作者：
PETTIJOHN, DE
DOI：
10.1099/00207713-47-2-510
发表时间：
1997-04-01
期刊：
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY
影响因子：
--
作者：
Rainey, FA;Nobre, MF;daCosta, MS
通讯作者：
daCosta, MS
耐辐射奇球菌锰外流蛋白作用的鉴定和评价
影响因子：
4.2
作者：
Sun H;Xu G;Zhan H;Chen H;Sun Z;Tian B;Hua Y
通讯作者：
Hua Y
影响因子：
64.8
作者：
Gourdon, Pontus;Liu, Xiang-Yu;Nissen, Poul
通讯作者：
Nissen, Poul