Identification and evaluation of the role of the manganese efflux protein in Deinococcus radiodurans.
Identification and evaluation of the role of the manganese efflux protein in Deinococcus radiodurans.
复制标题
耐辐射奇球菌锰外流蛋白作用的鉴定和评价
DOI：
10.1186/1471-2180-10-319
复制
发表时间：
2010-12-14
期刊：
影响因子：
4.2
通讯作者：
Hua Y
中科院分区：
文献类型：
--
作者：
Sun H;Xu G;Zhan H;Chen H;Sun Z;Tian B;Hua Y
背景
耐辐射异常球菌积累高水平的锰离子，这被认为与该微生物的耐辐射能力有关。然而，锰离子稳态的维持在D。抗辐射剂仍有待研究。
结果
在本研究中，我们鉴定了锰外排蛋白（MntE）。耐辐射的mntE的无效突变体比野生型菌株对锰离子更敏感，并且mntE突变体的生长在补锰培养基中延迟。此外，体内锰离子浓度显著增加。与这些特征一致，mntE突变体对H2O2、紫外线和γ辐射具有更强的抗性。突变株的胞内蛋白质氧化（羰基化）水平显着低于野生型菌株。
结论
我们的研究结果表明，dr1236确实是一个mntE同源，是必不可少的维持锰稳态在D。耐辐射的这些数据也为胞内锰离子参与D.耐辐射的
Background
Deinococcus radiodurans accumulates high levels of manganese ions, and this is believed to be correlated with the radiation resistance ability of this microorganism. However, the maintenance of manganese ion homeostasis in D. radiodurans remains to be investigated.
Results
In this study, we identified the manganese efflux protein (MntE) in D. radiodurans. The null mutant of mntE was more sensitive than the wild-type strain to manganese ions, and the growth of the mntE mutant was delayed in manganese-supplemented media. Furthermore, there was a substantial increase in the in vivo concentration of manganese ions. Consistent with these characteristics, the mntE mutant was more resistant to H2O2, ultraviolet rays, and γ-radiation. The intracellular protein oxidation (carbonylation) level of the mutant strain was remarkably lower than that of the wild-type strain.
Conclusions
Our results indicated that dr1236 is indeed a mntE homologue and is indispensable for maintaining manganese homeostasis in D. radiodurans. The data also provide additional evidence for the involvement of intracellular manganese ions in the radiation resistance of D. radiodurans.
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影响因子：
9.8
作者：
Daly MJ;Gaidamakova EK;Matrosova VY;Vasilenko A;Zhai M;Leapman RD;Lai B;Ravel B;Li SM;Kemner KM;Fredrickson JK
通讯作者：
Fredrickson JK
影响因子：
3.9
作者：
Al-Maghrebi, M;Fridovich, I;Benov, L
通讯作者：
Benov, L
DOI：
10.1073/pnas.78.2.1001
发表时间：
1981-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
REPINE, JE;PFENNINGER, OW;PETTIJOHN, DE
通讯作者：
PETTIJOHN, DE
DOI：
10.1099/00207713-47-2-510
发表时间：
1997-04-01
期刊：
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY
影响因子：
--
作者：
Rainey, FA;Nobre, MF;daCosta, MS
通讯作者：
daCosta, MS
影响因子：
3.6
作者：
McAllister, LJ;Tseng, HJ;Paton, JC
通讯作者：
Paton, JC