Transcriptional profiling in response to terminal drought stress reveals differential responses along the wheat genome.
Transcriptional profiling in response to terminal drought stress reveals differential responses along the wheat genome.
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DOI：
10.1186/1471-2164-10-279
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发表时间：
2009-06-24
期刊：
影响因子：
4.4
通讯作者：
Cattivelli L
中科院分区：
文献类型：
--
作者：
Aprile A;Mastrangelo AM;De Leonardis AM;Galiba G;Roncaglia E;Ferrari F;De Bellis L;Turchi L;Giuliano G;Cattivelli L
灌浆期水分胁迫对籽粒产量有显著影响，导致胚乳细胞数量减少，从而降低了籽粒积累干物质的能力。以小麦品种“中国春”（CS）、“中国春”末端缺失系（CS_5AL-10）和硬粒小麦品种“Creso”为研究材料，对籽粒灌浆期遭受轻度和重度干旱胁迫后的转录谱进行了分析，以寻找小麦基因组不同区域差异胁迫响应的证据。通过对Creso， CS及其缺失系的转录组分析，发现了8,552个不同表达水平的非冗余探针集，这主要是由于两个物种之间的比较。干旱处理改变了3056个探针组的表达。除了在Creso和CS中显示相似干旱响应的一组基因外，聚类分析还揭示了Creso、CS和CS_5AL-10不同基因组结构的干旱响应特征。与CS相比，一些干旱相关基因在Creso（缺乏D基因组）或CS_5AL-10缺失系中表达水平较低（或不表达）。其中一组基因的染色体位置通过基于pcr的D基因组（或5AL-10区域）定位得到证实。Creso、CS和CS_AL-10的许多基因簇的表达水平不同，表明这三种基因型的不同基因组组织可能影响植物对逆境的适应。将具有相似表达趋势的聚类进行分组和功能分类，以挖掘其激活或抑制的生物学平均值。ABA、脯氨酸、甘氨酸-甜菜碱和山梨糖醇通路相关基因在干旱胁迫下表达上调。此外，在CS_5AL-10中检测到一组转座子和反转录转座子的表达增强。面包小麦和硬粒小麦基因型对水分胁迫的生理反应不同，分子反应也有很大不同。基因组组织解释了位于D基因组上或由位于D基因组上的调节因子控制的数百个基因表达水平的差异。当基因组应激（染色体区域缺失）与低水分可用性相结合时，观察到基于转座子和反转录转座子激活的分子反应。
Water stress during grain filling has a marked effect on grain yield, leading to a reduced endosperm cell number and thus sink capacity to accumulate dry matter. The bread wheat cultivar Chinese Spring (CS), a Chinese Spring terminal deletion line (CS_5AL-10) and the durum wheat cultivar Creso were subjected to transcriptional profiling after exposure to mild and severe drought stress at the grain filling stage to find evidences of differential stress responses associated to different wheat genome regions. The transcriptome analysis of Creso, CS and its deletion line revealed 8,552 non redundant probe sets with different expression levels, mainly due to the comparisons between the two species. The drought treatments modified the expression of 3,056 probe sets. Besides a set of genes showing a similar drought response in Creso and CS, cluster analysis revealed several drought response features that can be associated to the different genomic structure of Creso, CS and CS_5AL-10. Some drought-related genes were expressed at lower level (or not expressed) in Creso (which lacks the D genome) or in the CS_5AL-10 deletion line compared to CS. The chromosome location of a set of these genes was confirmed by PCR-based mapping on the D genome (or the 5AL-10 region). Many clusters were characterized by different level of expression in Creso, CS and CS_AL-10, suggesting that the different genome organization of the three genotypes may affect plant adaptation to stress. Clusters with similar expression trend were grouped and functional classified to mine the biological mean of their activation or repression. Genes involved in ABA, proline, glycine-betaine and sorbitol pathways were found up-regulated by drought stress. Furthermore, the enhanced expression of a set of transposons and retrotransposons was detected in CS_5AL-10. Bread and durum wheat genotypes were characterized by a different physiological reaction to water stress and by a substantially different molecular response. The genome organization accounted for differences in the expression level of hundreds of genes located on the D genome or controlled by regulators located on the D genome. When a genomic stress (deletion of a chromosomal region) was combined with low water availability, a molecular response based on the activation of transposons and retrotransposons was observed.
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影响因子：
14.9
作者：
Kanehisa M;Araki M;Goto S;Hattori M;Hirakawa M;Itoh M;Katayama T;Kawashima S;Okuda S;Tokimatsu T;Yamanishi Y
通讯作者：
Yamanishi Y
DOI：
10.1080/14620316.2007.11512219
发表时间：
2007-03-01
影响因子：
1.9
作者：
Duangsrisai, S.;Yamada, K.;Yamaki, S.
通讯作者：
Yamaki, S.
DOI：
10.1073/pnas.0406069101
发表时间：
2004-10-19
影响因子：
11.1
作者：
Cook, D;Fowler, S;Thomashow, MF
通讯作者：
Thomashow, MF
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y
影响因子：
11.6
作者：
Abe, H;Urao, T;Yamaguchi-Shinozaki, K
通讯作者：
Yamaguchi-Shinozaki, K