Chemical coating polysaccharide on gigaporous polystyrene microspheres as a high-speed protein chromatography matrix
Chemical coating polysaccharide on gigaporous polystyrene microspheres as a high-speed protein chromatography matrix
复制标题
化学包覆多糖在大孔聚苯乙烯微球上作为高速蛋白质层析基质
DOI：
10.1002/pen.24376
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发表时间：
2016-12
影响因子：
3.2
通讯作者：
Jianguo Liu
中科院分区：
文献类型：
--
作者：
Jianbo Qu;Yuliang Xu;Junyi Liu;Shihai Li;Weiqing Zhou;Jianguo Liu
通过一种简单的方法直接化学接枝葡聚糖，实现了多糖在多孔聚苯乙烯（PS）微球上的永久性包覆，减少了蛋白质的非特异性吸附，并在微球上提供了易于衍生化的基团。首先通过乙酰化反应制备了氯乙酰化聚苯乙烯微球（PS-Cl），然后通过威廉姆森反应将亲水性葡聚糖直接接枝到疏水性聚苯乙烯微球上（PS-Dex）。均匀的侧结合葡聚糖涂层具有掩蔽PS微球的疏水表面的优点，同时保持其原始的巨孔结构。接枝后，PS微球的疏水表面被有效地掩蔽，非特异性蛋白质吸附大大减少。此外，富含羟基的涂层可以通过经典的化学方法容易地衍生化。填充PS-Dex的柱在高达3612 cm/h的高流速下呈现出良好的渗透性和机械强度。这些结果表明，PS-Dex是一种理想的高速蛋白质层析基质。Polym.工程科学，2016.© 2016塑料工程师协会
Permanent polysaccharide coating on gigaporous polystyrene (PS) microspheres was achieved through direct chemical grafting of dextran via a facile method, minimizing nonspecific adsorption of proteins and providing easily derivatizable groups on microspheres. Chloroacetylated PS microspheres (PS-Cl) were first developed through acetylation, and hydrophilic dextran was directly grafted to hydrophobic PS-Cl via a Williamson reaction (PS-Dex). The homogeneous side-bound dextran coating has the advantage of masking the hydrophobic surface of the PS microspheres, while maintains their original gigaporous structure. After grafting, the hydrophobic surface of the PS microspheres was effectively masked, and nonspecific protein adsorption was greatly reduced. Moreover, the hydroxyl-rich coating can be easily derivatized through classical chemical methods. A column packed with PS-Dex presented both good permeability and mechanical strength at high flow velocities of up to 3612 cm/h. These results implied that PS-Dex should be an ideal matrix for high-speed protein chromatography. POLYM. ENG. SCI., 2016. © 2016 Society of Plastics Engineers
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影响因子：
6.2
作者：
Jian-Bo Qu;Yan-Li Chen;Guan-Sheng Huan;Weiqing Zhou;Jian-guo Liu;Hu Zhu;Xiao-yun Zhang
通讯作者：
Jian-Bo Qu;Yan-Li Chen;Guan-Sheng Huan;Weiqing Zhou;Jian-guo Liu;Hu Zhu;Xiao-yun Zhang
影响因子：
7.4
作者：
Xian Huang;S. Zhang;G. Schultz;J. Henion
通讯作者：
Xian Huang;S. Zhang;G. Schultz;J. Henion
影响因子：
4.1
作者：
Y. Yang;M. Verzele
通讯作者：
Y. Yang;M. Verzele
DOI：
10.1016/s0021-9673(98)00068-5
发表时间：
1998-05
期刊：
Journal of chromatography. A
影响因子：
--
作者：
D. Whitney;M. Mccoy;N. Gordon;N. Afeyan
通讯作者：
D. Whitney;M. Mccoy;N. Gordon;N. Afeyan
影响因子：
4.1
作者：
S. Fulton;N. Afeyan;N. Gordon;F. Regnier
通讯作者：
S. Fulton;N. Afeyan;N. Gordon;F. Regnier