Maternal exposure to TCDD during gestation advanced sensory-motor development, but induced impairments of spatial learning and memory in adult male rat offspring.
Maternal exposure to TCDD during gestation advanced sensory-motor development, but induced impairments of spatial learning and memory in adult male rat offspring.
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母亲在妊娠期间接触 TCDD 会促进感觉运动发育，但会导致成年雄性大鼠后代的空间学习和记忆受损。
DOI：
10.1016/j.chemosphere.2018.08.118
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发表时间：
2018-12
期刊：
影响因子：
8.8
通讯作者：
Tang Nai Jun
中科院分区：
文献类型：
--
作者：
Zhang Hua Jing;Liu Ya Nan;Xian Ping;Ma Jing;Sun Ya Wen;Chen Jing Shan;Chen Xi;Tang Nai Jun
2,3,7,8-四氯二苯并-对二恶英（TCDD）是一种具有高持久性的内分泌干扰化学物质。即使是少量的也能在妊娠期通过胎盘屏障。接触TCDD会损害儿童神经系统的发育，导致晚年学习能力受损。但TCDD对婴幼儿神经行为发育的影响尚不清楚。妊娠大鼠在妊娠第8-14天（GD 8-14）连续给予TCDD（200或800 ng/day/kg）或等量的灌胃作为产前TCDD暴露模型。在后代中，在出生后第5天（PND5）评估早期神经行为发育，并从PND10开始监测睁眼情况。采用Morris水迷宫法对成年雄性子代进行空间记忆和学习能力评价。采用海马尼氏染色法和星形胶质细胞GFAP免疫组化法评价星形胶质细胞活性。行为测试结果显示，妊娠期接触TCDD会导致成年雄性后代运动活动过早和睁眼早，但会导致空间记忆和学习能力的严重缺陷。海马CA1区神经元形态和数量未受影响，但该区域星形胶质细胞活性明显降低。这些数据表明，围产期接触TCDD会导致成年雄性大鼠后代神经行为发育过早，但会损害其空间学习和记忆能力。海马星形胶质细胞活性降低可能在这些不良反应中起作用。
2,3,7,8-Tetrachlorodibenzo-p-Dioxin (TCDD) is an endocrine disrupting chemical (EDC) with high persistency. Even a low amount can pass the placental barrier during gestational exposure. Exposure to TCDD exposure can impair the development of the nervous system in children, leading to impaired learning ability in later-life. But the changes in neurobehavioral developments in infancy and childhood caused by TCDD are unknown. Pregnant Sprague-Dawley rats were given a consecutive daily dose of TCDD (200 or 800 ng/day/kg) or an equivalent volume of vehicle by gavage on gestational days 8–14 (GD 8–14) as the prenatal TCDD exposure model. In the offspring, early neurobehavioral development was assessed at postnatal day 5 (PND5) and eye-opening was monitored from PND10 onwards. Adult male offspring was tested by Morris Water Maze for spatial memory and learning ability evaluation. Hippocampus Nissl's staining and astrocyte GFAP immunohistochemistry were used to evaluate the activity of astrocytes. The results of the behavioral tests showed that gestational TCDD exposure induced premature motor activity and earlier eyes-opening, but lead to serious deficits of spatial memory and learning ability in the adult male offspring. Morphology and number of neurons in the hippocampus CA1 region was not affected, while the activity of astrocytes in the same region was significantly reduced. These data indicate that perinatal TCDD exposure induced premature neurobehavioral development but impaired the spatial learning and memory in adult male rat offspring. The decreased activity of astrocytes in the hippocampus may play a role in these adverse effects.
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影响因子：
--
作者：
Schousboe A;Scafidi S;Bak LK;Waagepetersen HS;McKenna MC
通讯作者：
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4
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作者：
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