More Than Gliding: Involvement of GldD and GldG in the Virulence of Flavobacterium psychrophilum.
More Than Gliding: Involvement of GldD and GldG in the Virulence of Flavobacterium psychrophilum.
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DOI：
10.3389/fmicb.2017.02168
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发表时间：
2017
影响因子：
5.2
通讯作者：
Duchaud E
中科院分区：
文献类型：
--
作者：
Pérez-Pascual D;Rochat T;Kerouault B;Gómez E;Neulat-Ripoll F;Henry C;Quillet E;Guijarro JA;Bernardet JF;Duchaud E
黄杆菌属的大多数成员的一个迷人的特征是它们通过滑动运动在表面上移动的能力。嗜冷黄杆菌（Flavobacterium psychrophilum）是世界范围内养殖鲑鱼的重要病原体，其基因组中含有19个gld和spr基因，这些基因被证明是约翰逊黄杆菌（Flavobacterium johnsoniae）中滑行或传播所必需的;然而，它们在其生活方式中的相对作用仍然未知。为了解决这个问题，在F.嗜冷菌菌株THCO 2 -90。将转座子插入gldD和gldG基因中。虽然野生型菌株是精通粘附，生物膜形成，并显示出强大的蛋白水解活性，这两个突变体失去了这些特性。细胞外蛋白质组的比较揭示了两种突变体的重要修改，与可能通过外膜运输的IX型分泌系统的蛋白质的量的显着减少，表明GldD和GldG蛋白质是需要一个有效的活性，该系统。此外，使用虹鳟鱼浴和注射感染模型观察到毒力显着下降。我们的研究结果揭示了gldD和gldG基因的其他作用，这些基因可能对F。嗜冷菌的生活方式，包括毒力。
A fascinating characteristic of most members of the genus Flavobacterium is their ability to move over surfaces by gliding motility. Flavobacterium psychrophilum, an important pathogen of farmed salmonids worldwide, contains in its genome the 19 gld and spr genes shown to be required for gliding or spreading in Flavobacterium johnsoniae; however, their relative role in its lifestyle remains unknown. In order to address this issue, two spreading deficient mutants were produced as part of a Tn4351 mutant library in F. psychrophilum strain THCO2-90. The transposons were inserted in gldD and gldG genes. While the wild-type strain is proficient in adhesion, biofilm formation and displays strong proteolytic activity, both mutants lost these characteristics. Extracellular proteome comparisons revealed important modifications for both mutants, with a significant reduction of the amounts of proteins likely transported through the outer membrane by the Type IX secretion system, indicating that GldD and GldG proteins are required for an effective activity of this system. In addition, a significant decrease in virulence was observed using rainbow trout bath and injection infection models. Our results reveal additional roles of gldD and gldG genes that are likely of importance for the F. psychrophilum lifestyle, including virulence.
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