Wood Formation in Trees Is Increased by Manipulating PXY-Regulated Cell Division.
Wood Formation in Trees Is Increased by Manipulating PXY-Regulated Cell Division.
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DOI：
10.1016/j.cub.2015.02.023
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发表时间：
2015-04-20
期刊：
影响因子：
9.2
通讯作者：
Turner, Simon R.
中科院分区：
文献类型：
--
作者：
Etchells, J. Peter;Mishra, Laxmi S.;Kumar, Manoj;Campbell, Liam;Turner, Simon R.
The woody tissue of trees is composed of xylem cells that arise from divisions of stem cells within the cambial meristem. The rate of xylem cell formation is dependent upon the rate of cell division within the cambium and is controlled by both genetic and environmental factors. In the annual plant Arabidopsis, signaling between a peptide ligand CLE41 and a receptor kinase PXY controls cambial cell divisions; however, the pathway regulating secondary growth in trees has not been identified. Here, we show that an aspen receptor kinase PttPXY and its peptide ligand PttCLE41 are functional orthologs and act to control a multifunctional pathway that regulates both the rate of cambial cell division and woody tissue organization. Ectopic overexpression of PttPXY and PttCLE41 genes in hybrid aspen resulted in vascular tissue abnormalities and poor plant growth. In contrast, precise tissue-specific overexpression generated trees that exhibited a 2-fold increase in the rate of wood formation, were taller, and possessed larger leaves compared to the controls. Our results demonstrate that the PXY-CLE pathway has evolved to regulate secondary growth and manipulating this pathway can result in dramatically increased tree growth and productivity. PXY receptor kinase and CLE peptide signal to regulate radial growth in trees Engineering PXY-CLE expression can lead to large increases in wood formation Manipulating PXY-CLE also leads to increased tree height and leaf size The results can be used to generate trees that are more productive Etchells et al. show that altering the expression of the poplar homologs of the receptor kinase PXY and its peptide ligand CLE41 results in increased cambial cell division in hybrid aspen. 2-fold increases in the rate of wood formation were observed, demonstrating that engineering PXY/CLE41 signaling offers a means to increase tree productivity.
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影响因子：
12.3
作者：
Carpenter AE;Jones TR;Lamprecht MR;Clarke C;Kang IH;Friman O;Guertin DA;Chang JH;Lindquist RA;Moffat J;Golland P;Sabatini DM
通讯作者：
Sabatini DM
影响因子：
56.9
作者：
Kondo, Tatsuhiko;Sawa, Shinichiro;Sakagami, Youji
通讯作者：
Sakagami, Youji
影响因子：
9.2
作者：
Fisher, Kate;Turner, Simon
通讯作者：
Turner, Simon
影响因子：
56.9
作者：
Tuskan, G. A.;DiFazio, S.;Rokhsar, D.
通讯作者：
Rokhsar, D.
影响因子：
5.1
作者：
Nilsson, O;Little, CHA;Olsson, O
通讯作者：
Olsson, O