Embedding matrix for simultaneous processing of multiple histological samples.

Embedding matrix for simultaneous processing of multiple histological samples.
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DOI:
10.1016/j.jneumeth.2012.06.005
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发表时间:
2012-07-30
影响因子:
3
通讯作者:
Bleiwas, Cynthia
Bleiwas, Cynthia
中科院分区:
医学4区
文献类型:
--
作者:
Smiley, John F.;Bleiwas, Cynthia

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描述了一种用于同时处理多个固定组织样本的组织学方法。组织样品被嵌入明胶-白蛋白蛋白基质中,该基质被快速固化并使用交联剂结合到组织表面。冰冻切片后,可对含有多个样本的单个切片进行免疫细胞化学和组织化学染色。该方法被用于同时处理多个啮齿类动物的脑,并用于重建人类尸检的碎片化脑样本。
A method is described for simultaneous histological processing of multiple fixed tissue samples. The tissue samples are embedded in a gelatin-albumin protein matrix that is rapidly solidified and bound to the tissue surface using a cross-linking reagent. After freezing and sectioning, the individual sections containing multiple samples can be processed for immunocytochemical and histochemical staining. The method is demonstrated for simultaneous processing of multiple rodent brains, and for reconstruction of fragmented human postmortem brain samples.
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发表时间: 1998-07-01
期刊: NATURE MEDICINE
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