MultiEditR: The first tool for the detection and quantification of RNA editing from Sanger sequencing demonstrates comparable fidelity to RNA-seq.
MultiEditR: The first tool for the detection and quantification of RNA editing from Sanger sequencing demonstrates comparable fidelity to RNA-seq.
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MultiEditR：第一个用于检测和定量来自桑格测序的RNA编辑的工具，显示出与RNA-seq相当的保真度。
DOI：
10.1016/j.omtn.2021.07.008
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发表时间：
2021-09-03
期刊：
影响因子：
--
通讯作者：
Pecori R
中科院分区：
文献类型：
--
作者：
Kluesner MG;Tasakis RN;Lerner T;Arnold A;Wüst S;Binder M;Webber BR;Moriarity BS;Pecori R
我们提出了MultiEditR（通过R中痕迹推理的多重编辑去卷积），这是第一个专门设计用于检测和定量来自桑格测序的RNA编辑的算法（z.umn.edu/multieditr）。尽管RNA编辑通常通过测量来自桑格测序迹线的峰的高度来评估，但该方法的准确性和精确度尚未针对金标准下一代测序方法进行评估。通过与RNA测序（RNA-seq）和基于扩增子的深度测序的全面比较，我们表明MultiEditR在检测内源性和可编程RNA编辑方面是准确、精确和可靠的。RNA编辑允许细胞瞬时重写遗传密码。然而，检测和定量RNA编辑依赖于RNA-seq，其昂贵且局部分辨率差。在这里，我们开发了MultiEditR（https：//moriaritylab.shinyapps.io/multieditr/）来分析来自桑格测序的RNA编辑，其成本仅为一小部分，同时保持与RNA-seq相当的保真度。
We present MultiEditR (Multiple Edit Deconvolution by Inference of Traces in R), the first algorithm specifically designed to detect and quantify RNA editing from Sanger sequencing (z.umn.edu/multieditr). Although RNA editing is routinely evaluated by measuring the heights of peaks from Sanger sequencing traces, the accuracy and precision of this approach has yet to be evaluated against gold standard next-generation sequencing methods. Through a comprehensive comparison to RNA sequencing (RNA-seq) and amplicon-based deep sequencing, we show that MultiEditR is accurate, precise, and reliable for detecting endogenous and programmable RNA editing. RNA editing allows cells to transiently rewrite the genetic code. However, detecting and quantifying RNA editing relies on RNA-seq, which is expensive and suffers from poor local resolution. Here, we develop MultiEditR (https://moriaritylab.shinyapps.io/multieditr/) to analyze RNA editing from Sanger sequencing for a fraction of the cost while maintaining a comparable fidelity to RNA-seq.
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影响因子：
9.5
作者：
Diroma, Maria Angela;Ciaccia, Loredana;Picardi, Ernesto
通讯作者：
Picardi, Ernesto
影响因子：
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DOI：
10.1002/dvdy.24183
发表时间：
2014-12
期刊：
Developmental dynamics : an official publication of the American Association of Anatomists
影响因子：
--
作者：
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通讯作者：
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影响因子：
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作者：
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