Covalent surface modification of a titanium alloy with a phosphorylcholine-containing copolymer for reduced thrombogenicity in cardiovascular devices.
Covalent surface modification of a titanium alloy with a phosphorylcholine-containing copolymer for reduced thrombogenicity in cardiovascular devices.
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DOI：
10.1002/jbm.a.32184
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发表时间：
2009-10
影响因子：
4.9
通讯作者：
Wagner, William R.
中科院分区：
文献类型：
--
作者：
Ye, Sang-Ho;Johnson, Carl A.;Woolley, Joshua R.;Snyder, Trevor A.;Gamble, Lara J.;Wagner, William R.
关键词：
我们的目标是开发一种钛合金(TiAl6V4)的表面改性策略，以在严格的血液接触心血管应用中为表面提供抗血栓性能，例如在心脏辅助装置中发现的表面。我们假设这可以通过磷脂聚合物聚(2-甲基丙烯酰氧乙基磷酰胆碱(MPC)-甲基丙烯酸共聚物)(PMA)的共价连接来完成。TiAl6V4用射频辉光放电处理H2O等离子体，用3-氨基丙基三乙氧基硅烷(APS)硅烷化，再用氨等离子体处理，以提高表面反应活性。然后，通过TiAl6V4表面的氨基与PMA表面的羧基之间的缩合反应，用PMA对TiAl6V4表面进行改性。用X射线光电子能谱证实了TiAl6V4的表面组成，证实了APS和PMA成功地对TiAl6V4表面进行了改性，表面硅和磷的增加证明了这一点。水和氨的等离子体处理有效地进一步提高了TiAl6V4的表面反应性，表面PMA增加了。绵羊纤维蛋白原在PMA修饰的表面上的吸附比未修饰的表面减少，通过摇摆试验在体外与绵羊血液的接触显示，与未修饰的TiAl6V4和聚苯乙烯对照相比，血小板沉积和整体相血小板激活显著减少。结果表明，TiAl6V4表面的PMA改性方案为提高心血管设备血液接触表面的抗血栓性能提供了一条潜在的途径。
Our objective was to develop a surface modification strategy for a titanium alloy (TiAl6V4) to provide thromboresistance for surfaces in rigorous blood-contacting cardiovascular applications, such as that found in ventricular assist devices. We hypothesized that this could be accomplished by the covalent attachment of a phospholipid polymer, poly(2-methacryloyloxyethylphosphorylcholine (MPC)-co-methacryl acid) (PMA). TiAl6V4 was H2O plasma treated by radio frequency glow discharge, silanated with 3-aminopropyltriethoxysilane (APS), and ammonia plasma treated to increase surface reactivity. The TiAl6V4 surface was then modified with PMA via a condensation reaction between the amino groups on the TiAl6V4 surface and the carboxyl groups on PMA. The surface composition was verified by X-ray photoelectron spectroscopy, confirming successful modification of the TiAl6V4 surfaces with APS and PMA as evidenced by increased Si and P. Plasma treatments with H2O and ammonia were effective at further increasing the surface reactivity of TiAl6V4 as evidenced by increased surface PMA. The adsorption of ovine fibrinogen onto PMA-modified surfaces was reduced relative to unmodified surfaces, and in vitro ovine blood contact through a rocking test revealed marked reductions in platelet deposition and bulk phase platelet activation relative to unmodified TiAl6V4 and polystyrene controls. The results indicate that the PMA-modification scheme for TiAl6V4 surfaces offers a potential pathway to improve the thromboresistance of the blood-contacting surfaces of cardiovascular devices.
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影响因子：
14
作者：
Hasegawa, T;Iwasaki, Y;Ishihara, K
通讯作者：
Ishihara, K
影响因子：
9.9
作者：
Daniels, MW;Sefcik, J;McCormick, AV
通讯作者：
McCormick, AV
影响因子：
3.9
作者：
Holmlin, RE;Chen, XX;Whitesides, GM
通讯作者：
Whitesides, GM
影响因子：
3.3
作者：
Chevallier, P;Castonguay, N;Laroche, G
通讯作者：
Laroche, G
DOI：
10.1016/s1468-6996(00)00012-7
发表时间：
2000-01-01
影响因子：
5.5
作者：
Ishihara, K.
通讯作者：
Ishihara, K.