The Entomopathogenic Fungi Isaria fumosorosea Plays a Vital Role in Suppressing the Immune System of Plutella xylostella: RNA-Seq and DGE Analysis of Immunity-Related Genes.
The Entomopathogenic Fungi Isaria fumosorosea Plays a Vital Role in Suppressing the Immune System of Plutella xylostella: RNA-Seq and DGE Analysis of Immunity-Related Genes.
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昆虫病原真菌 Isaria fumosorosea 在抑制小菜蛾免疫系统中发挥重要作用：免疫相关基因的 RNA-Seq 和 DGE 分析
DOI：
10.3389/fmicb.2017.01421
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发表时间：
2017
影响因子：
5.2
通讯作者：
Jin F
中科院分区：
文献类型：
--
作者：
Xu J;Xu X;Shakeel M;Li S;Wang S;Zhou X;Yu J;Xu X;Yu X;Jin F
大多数昆虫病原真菌已被用作替代控制剂，以降低昆虫抗性和杀虫剂对环境的有害影响。其中，玫烟色棒束孢在防治各种害虫方面也显示出巨大的潜力。本研究探讨了小菜蛾对小菜蛾I.利用RNA测序和差异表达技术在基因组水平上对不同时间点的玫烟色孢进行了初步的研究。为了深入了解宿主-病原体在基因组水平上的相互作用，构建了感染后12、18、24和36 h的小菜蛾幼虫和对照的5个文库。总共鉴定了161个免疫相关基因，并将其分为四类：免疫识别家族、Toll和Imd通路、黑化和抗菌肽（AMP）。免疫相关基因的差异表达结果显示，在处理后12、18、24和36 h，小菜蛾中分别有15、13、53和14个基因表达上调，38、51、56和49个基因表达下调。免疫相关基因的RNA-Seq结果显示，I.烟色的为了通过RT-qPCR验证RNA-Seq结果，随机选择22个免疫相关基因。总之，我们的结果表明，I。玫烟色孢菌具有抑制小菜蛾免疫反应的潜力，可作为防治小菜蛾的潜在生物农药。
Most, if not all, entomopathogenic fungi have been used as alternative control agents to decrease the insect resistance and harmful effects of the insecticides on the environment. Among them, Isaria fumosorosea has also shown great potential to control different insect pests. In the present study, we explored the immune response of P. xylostella to the infection of I. fumosorosea at different time points by using RNA-Sequencing and differential gene expression technology at the genomic level. To gain insight into the host-pathogen interaction at the genomic level, five libraries of P. xylostella larvae at 12, 18, 24, and 36 h post-infection and a control were constructed. In total, 161 immunity-related genes were identified and grouped into four categories; immune recognition families, toll and Imd pathway, melanization, and antimicrobial peptides (AMPs). The results of differentially expressed immunity-related genes depicted that 15, 13, 53, and 14 up-regulated and 38, 51, 56, and 49 were down-regulated in P. xylostella at 12, 18, 24, and 36 h post-treatment, respectively. RNA-Seq results of immunity-related genes revealed that the expression of AMPs was reduced after treatment with I. fumosorosea. To validate RNA-Seq results by RT-qPCR, 22 immunity-related genes were randomly selected. In conclusion, our results demonstrate that I. fumosorosea has the potential to suppress the immune response of P. xylostella and can become a potential biopesticide for controlling P. xylostella.
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影响因子：
6.7
作者：
Abkallo HM;Tangena JA;Tang J;Kobayashi N;Inoue M;Zoungrana A;Colegrave N;Culleton R
通讯作者：
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4
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影响因子：
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作者：
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通讯作者：
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