Transcript and protein profiling analysis of the Destruxin A-induced response in larvae of Plutella xylostella.
Transcript and protein profiling analysis of the Destruxin A-induced response in larvae of Plutella xylostella.
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DOI：
10.1371/journal.pone.0060771
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Ren S
中科院分区：
文献类型：
--
作者：
Han P;Jin F;Dong X;Fan J;Qiu B;Ren S
破坏素是由金龟子绿僵菌、座壳孢菌、芸苔链格孢菌和泡丝球腔菌等昆虫病原真菌产生的真菌毒素。它可以影响昆虫的多种生物学过程，包括天然免疫、细胞内Ca 2+通道和细胞凋亡，并且具有剂量依赖性。Dtxs作为生物防治剂已经使用了很长时间，然而，其作用的分子机制仍然是未知的。本研究采用数字基因表达（DGE）和双向电泳（2-DE）技术研究了dtx A对小菜蛾（Plutella xyllostella（L.）幼虫通过DGE和2-DE分析，1584个基因和42个蛋白点被鉴定为响应dtx A而上调或下调至少2倍。首先，注射dtx A促进了肽聚糖识别蛋白（PGRP）的增加，从而激活了Toll信号通路，诱导抗菌物质如天蚕素和葛兰素的产生。Dtx A还刺激在先天免疫中起重要作用的酚氧化酶原（proPO）系统，并导致外部生物体的黑化。其次，dtx A抑制Toll通路相关基因的表达，诱导丝氨酸蛋白酶抑制剂（serpins）的表达，尤其是serpin 2的表达，阻断了proPO系统的过程。此外，dtx A还参与了外源性物质的解毒、细胞凋亡、钙信号通路和昆虫激素合成等生理过程。转录和蛋白质谱分析将提供对小菜蛾幼虫响应dtx A的潜在分子作用机制的深入了解。
Destruxins (dtxs) are the mycotoxin produced by certain entomopathogenic fungi, such as Metarhizium anisopliae, Aschersonia sp, Alternaria brassicae and Ophiosphaerella herpotrichae. It can affect a wide variety of biological processes in insects, including innate immune, Ca2+ channel in cells, and apoptosis in a dose-dependent manner. Dtxs have been used as biological control agent for a long time, however, their molecular mechanism of action is still unknown. In this study, both digital gene expression (DGE) and two-dimensional electrophoresis (2-DE) approaches were adopted to examine the effects of dtx A on Plutella xyllostella (L.) larvae. By using DGE and 2-DE analyses, 1584 genes and 42 protein points were identified as being up- or down regulated at least 2-fold in response to dtx A. Firstly, injection of dtx A to larvae accelerated the increase of peptidoglycan recognition protein (PGRP), which could activate the Toll signal pathway inducing production of antibacterial substances such as cecropin and gloverin. Dtx A also stimulated prophenoloxidase (proPO) system which plays an important role in innate immunity and leads to melanization of external organisms. Secondly, dtx A suppressed the expression of genes related to the Toll pathway, and induced expression of serine proteinase inhibitors (serpins), especially the serpin 2 that blocked process of the proPO system. Finally, other physiological process like xenobiotics detoxification, apoptosis, calcium signaling pathway and insect hormone biosynthesis, were also mediated in response to dtx A toxicity. Transcript and protein profiling analyses will provide an insight into the potential molecular mechanism of action in P. xylostella larvae in response to dtx A.
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DOI：
10.1038/nri2569
发表时间：
2009-07
期刊：
Nature reviews. Immunology
影响因子：
--
作者：
Geijtenbeek TB;Gringhuis SI
通讯作者：
Gringhuis SI
影响因子：
3.1
作者：
Altincicek, Boran;Linder, Monica;Vilcinskas, Andreas
通讯作者：
Vilcinskas, Andreas
影响因子：
3.4
作者：
Karlhofer, Julia;Schafellner, Christa;Hoch, Gernot
通讯作者：
Hoch, Gernot
影响因子：
--
作者：
Amiri-Besheli, B;Khambay, B;Butt, TM
通讯作者：
Butt, TM
影响因子：
2.8
作者：
Hu, Qiong-Bo;Ren, Shun-Xiang;Musa, Peter D.
通讯作者：
Musa, Peter D.