Proteomics shows new faces for the old penicillin producer Penicillium chrysogenum.
Proteomics shows new faces for the old penicillin producer Penicillium chrysogenum.
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DOI：
10.1155/2012/105109
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发表时间：
2012
影响因子：
--
通讯作者：
García-Estrada C
中科院分区：
文献类型：
--
作者：
Barreiro C;Martín JF;García-Estrada C
真菌由大量微生物组成，包括子囊菌门(大多数已描述的真菌)、担子菌门(蘑菇或高等真菌)以及接合菌门和合并菌门(基础或低等真菌)，它们能产生工业上感兴趣的次生代谢物，如β-内酰胺类抗生素。这些化合物是世界上最常用的处方药之一。自从弗莱明80年前首次发现新青霉菌以来，青霉菌作为抗菌源的作用获得了专利。自从60年前分离出产黄青霉NRRL 1951以来，经典的诱变和筛选程序导致了生产率提高(至少三个数量级)的工业菌株的发展。新的“组学”时代为理解工业菌株改良过程的潜在机制提供了关键。对应用于产黄假单胞菌的不同蛋白质组学方法的回顾表明，这种微生物的工业化改造是几种代谢途径仔细重新平衡的结果。此外，对产黄假单胞菌的分泌组分析为这种多功能丝状真菌的新的工业应用打开了大门。
Fungi comprise a vast group of microorganisms including the Ascomycota (majority of all described fungi), the Basidiomycota (mushrooms or higher fungi), and the Zygomycota and Chytridiomycota (basal or lower fungi) that produce industrially interesting secondary metabolites, such as β-lactam antibiotics. These compounds are one of the most commonly prescribed drugs world-wide. Since Fleming's initial discovery of Penicillium notatum 80 years ago, the role of Penicillium as an antimicrobial source became patent. After the isolation of Penicillium chrysogenum NRRL 1951 six decades ago, classical mutagenesis and screening programs led to the development of industrial strains with increased productivity (at least three orders of magnitude). The new “omics” era has provided the key to understand the underlying mechanisms of the industrial strain improvement process. The review of different proteomics methods applied to P. chrysogenum has revealed that industrial modification of this microorganism was a consequence of a careful rebalancing of several metabolic pathways. In addition, the secretome analysis of P. chrysogenum has opened the door to new industrial applications for this versatile filamentous fungus.
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影响因子：
4.4
作者：
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通讯作者：
Lee YH
影响因子：
6.7
作者：
Bhadauria, Vijai;Zhao, Wen-Sheng;Peng, You-Liang
通讯作者：
Peng, You-Liang
影响因子：
2.9
作者：
Candiano, G;Bruschi, M;Righetti, PG
通讯作者：
Righetti, PG
影响因子：
3.2
作者：
Arias-Barrau, E;Olivera, ER;Miñambres, B
通讯作者：
Miñambres, B
DOI：
10.1073/pnas.92.13.6200
发表时间：
1995-06-20
影响因子：
11.1
作者：
FIERRO, F;BARREDO, JL;MARTIN, JF
通讯作者：
MARTIN, JF