Non-telecentric two-photon microscopy for 3D random access mesoscale imaging.
Non-telecentric two-photon microscopy for 3D random access mesoscale imaging.
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DOI：
10.1038/s41467-022-28192-0
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发表时间：
2022-01-27
影响因子：
16.6
通讯作者：
Baden T
中科院分区：
文献类型：
--
作者：
Janiak FK;Bartel P;Bale MR;Yoshimatsu T;Komulainen E;Zhou M;Staras K;Prieto-Godino LL;Euler T;Maravall M;Baden T
衍射限制双光子显微镜允许微创光学监测神经元活动。然而，大多数传统的双光子显微镜施加显着的约束的成像视场的大小和特定形状的有效激发体积，从而限制了范围内的生物问题，可以解决和可获得的信息。在这里，采用非远心光学设计，我们提出了一种低成本，易于实施和灵活的解决方案，以解决这些限制，提供了几倍的扩展三维视野。此外，经由电可调透镜的快速激光聚焦控制允许在三维中分离的远程区域的近同时成像，并且允许成像平面的弯曲以遵循生物结构中的自然曲率。至关重要的是，我们的核心设计可以在几个小时内轻松实现（和逆转），使其非常适合作为进一步开发的基础平台。我们展示了我们的系统在斑马鱼，小鼠和果蝇的各种例子中的神经元活动成像的应用。许多常规的双光子显微镜限制成像视场的大小和有效激发体积的特定形状。作者提出了一种低成本的非远心光学设计，该设计扩展了3D视场，并允许成像平面弯曲以遵循生物结构中的自然曲率。
Diffraction-limited two-photon microscopy permits minimally invasive optical monitoring of neuronal activity. However, most conventional two-photon microscopes impose significant constraints on the size of the imaging field-of-view and the specific shape of the effective excitation volume, thus limiting the scope of biological questions that can be addressed and the information obtainable. Here, employing a non-telecentric optical design, we present a low-cost, easily implemented and flexible solution to address these limitations, offering a several-fold expanded three-dimensional field of view. Moreover, rapid laser-focus control via an electrically tunable lens allows near-simultaneous imaging of remote regions separated in three dimensions and permits the bending of imaging planes to follow natural curvatures in biological structures. Crucially, our core design is readily implemented (and reversed) within a matter of hours, making it highly suitable as a base platform for further development. We demonstrate the application of our system for imaging neuronal activity in a variety of examples in zebrafish, mice and fruit flies. Many conventional two-photon microscopes restrict the size of the imaging field-of-view and the specific shape of the effective excitation volume. The authors present a low-cost non-telecentric optical design that expands the 3D field of view and permits the bending of imaging planes to follow natural curvatures in biological structures.
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影响因子：
48
作者：
Grewe, Benjamin F.;Langer, Dominik;Helmchen, Fritjof
通讯作者：
Helmchen, Fritjof
影响因子：
48
作者：
Ahrens, Misha B.;Orger, Michael B.;Keller, Philipp J.
通讯作者：
Keller, Philipp J.
影响因子：
4.5
作者：
Euler, Thomas;Hausselt, Susanne E.;Margolis, David J.;Breuninger, Tobias;Castell, Xavier;Detwiler, Peter B.;Denk, Winfried
通讯作者：
Denk, Winfried
影响因子：
3.4
作者：
Grewe BF;Voigt FF;van 't Hoff M;Helmchen F
通讯作者：
Helmchen F
影响因子：
3.4
作者：
Chong, Ee Zhuan;Panniello, Mariangela;Booth, Martin J.
通讯作者：
Booth, Martin J.