Triggering Growth via Growth Initiation Factors in Nature: A Putative Mechanism for in situ Cultivation of Previously Uncultivated Microorganisms.
Triggering Growth via Growth Initiation Factors in Nature: A Putative Mechanism for in situ Cultivation of Previously Uncultivated Microorganisms.
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通过自然界的生长引发因素触发生长：一种原位种植以前未经文化的微生物的假定机制。
DOI：
10.3389/fmicb.2021.537194
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发表时间：
2021
影响因子：
5.2
通讯作者：
Aoi Y
中科院分区：
文献类型：
--
作者：
Jung D;Machida K;Nakao Y;Kindaichi T;Ohashi A;Aoi Y
大多数微生物在标准实验室条件下无法培养。另一方面，在自然培养的膜结合装置中培养微生物（原位培养）可能是克服这一限制的有效方法。在本研究中，我们采用原位培养的方法分离了多种以前未培养的海洋海绵相关微生物，并与常规方法进行了比较分析。然后，我们试图通过原位培养来研究未培养微生物的关键和未知的生长机制，重点关注生长启动因子的生长触发。通过原位培养比标准直接电镀（SDP）分离出更多新的和多样化的微生物类型。我们假设一些抗培养的环境微生物处于非生长状态，需要生长启动因子才能恢复，而这些因子可以从环境中提供（在本研究中来自海绵）。根据假设，比较了原位培养和SDP培养菌株在琼脂培养基上的恢复效果。培养基中添加少量海绵提取物可提高原位菌株在饥饿恢复阶段的菌落形成效率，而对SDP菌株的菌落形成没有积极影响。相反，所有测试菌株的特定生长速率或饱和细胞密度均未受到积极影响。这些结果表明，(1)海绵提取物含有促进非生长微生物恢复的化合物，(2)这些物质对原位菌株起作用，(3)海绵提取物中的生长起始因子不是持续促进生长活性，而是作为再生的触发器（从非生长状态复苏）。
Most microorganisms resist cultivation under standard laboratory conditions. On the other hand, cultivating microbes in a membrane-bound device incubated in nature (in situ cultivation) can be an effective approach to overcome this limitation. In the present study, we applied in situ cultivation to isolate diverse previously uncultivated marine sponge-associated microbes and comparatively analyzed this method’s efficiencies with those of the conventional method. Then, we attempted to investigate the key and previously unidentified mechanism of growing uncultivated microorganisms by in situ cultivation focusing on growth triggering via growth initiation factor. Significantly more novel and diverse microbial types were isolated via in situ cultivation than by standard direct plating (SDP). We hypothesized that some of environmental microorganisms which resist cultivation are in a non-growing state and require growth initiation factors for the recovery and that these can be provided from the environment (in this study from marine sponge). According to the hypothesis, the effect of the sponge extract on recovery on agar medium was compared between strains derived from in situ and SDP cultivation. Adding small amounts of the sponge extracts to the medium elevated the colony-formation efficiencies of the in situ strains at the starvation recovery step, while it showed no positive effect on that of SDP strains. Conversely, specific growth rates or saturated cell densities of all tested strains were not positively affected. These results indicate that, (1) the sponge extract contains chemical compounds that facilitate recovery of non-growing microbes, (2) these substances worked on the in situ strains, and (3) growth initiation factor in the sponge extract did not continuously promote growth activity but worked as triggers for regrowth (resuscitation from non-growing state).
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影响因子：
--
作者：
D'Onofrio A;Crawford JM;Stewart EJ;Witt K;Gavrish E;Epstein S;Clardy J;Lewis K
通讯作者：
Lewis K
影响因子：
15.5
作者：
Fenn K;Strandwitz P;Stewart EJ;Dimise E;Rubin S;Gurubacharya S;Clardy J;Lewis K
通讯作者：
Lewis K
影响因子：
2.1
作者：
BRANTLEY, SE;MOLINSKI, TF;DELONG, EF
通讯作者：
DELONG, EF
影响因子：
4.2
作者：
Esteves, Ana I. S.;Cullen, Alescia;Thomas, Torsten
通讯作者：
Thomas, Torsten
DOI：
10.1073/pnas.1203287109
发表时间：
2012-07-03
影响因子：
11.1
作者：
Fan, Lu;Reynolds, David;Thomas, Torsten
通讯作者：
Thomas, Torsten