Biohydrogen production from arabinose and glucose using extreme thermophilic anaerobic mixed cultures.
Biohydrogen production from arabinose and glucose using extreme thermophilic anaerobic mixed cultures.
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DOI：
10.1186/1754-6834-5-6
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发表时间：
2012-02-13
影响因子：
6.3
通讯作者：
Alves MM
中科院分区：
文献类型：
--
作者：
Abreu AA;Karakashev D;Angelidaki I;Sousa DZ;Alves MM
利用有机农业和林业废物的第二代氢发酵技术正在出现。由木质纤维素材料的预处理产生的己糖和戊糖的有效微生物发酵对于这些过程的成功至关重要。在连续（70°C，pH 5.5）和分批（70 ° C，pH 5.5和pH 7）测定中研究了通过极端嗜热、厌氧、混合培养物将阿拉伯糖和葡萄糖转化为氢。两个膨胀颗粒污泥床（EGSB）反应器，Rarab和Rgluc，连续进料阿拉伯糖和葡萄糖，分别。阿拉伯糖和葡萄糖的有机负荷率（OLR）范围从4.3至7.1 kgCOD m-3 d-1的反应器性能没有显着差异。然而，对于14.2 kgCOD m-3 d-1的OLR，Rarab的产氢速率和产氢率高于Rgluc（Rarab和Rgluc的平均产氢速率分别为3.2和2.0 LH 2 L-1 d-1，产氢率分别为1.10和0.75 molH 2 mol-1底物）。在Rgluc中较低的氢产生与较高的乳酸产生相关。变性梯度凝胶电泳（DGGE）结果显示，细菌群落组成的运行期间和反应器之间没有显着差异。当氢分压保持较低时，在分批实验中观察到产氢增加，阿拉伯糖和葡萄糖都作为底物。当使用pH 7而不是pH 5.5时，糖被完全消耗并且氢产生被刺激（高62%）。在较高的有机负荷下，阿拉伯糖的连续产氢速率显著高于葡萄糖。氢分压对分批模式下葡萄糖制氢的影响与糖的利用程度有关，而与底物转化为氢的效率无关。此外，在pH 7.0，糖的吸收，产氢和产量高于在pH 5.5，与阿拉伯糖和葡萄糖作为底物。
Second generation hydrogen fermentation technologies using organic agricultural and forestry wastes are emerging. The efficient microbial fermentation of hexoses and pentoses resulting from the pretreatment of lingocellulosic materials is essential for the success of these processes. Conversion of arabinose and glucose to hydrogen, by extreme thermophilic, anaerobic, mixed cultures was studied in continuous (70°C, pH 5.5) and batch (70°C, pH 5.5 and pH 7) assays. Two expanded granular sludge bed (EGSB) reactors, Rarab and Rgluc, were continuously fed with arabinose and glucose, respectively. No significant differences in reactor performance were observed for arabinose and glucose organic loading rates (OLR) ranging from 4.3 to 7.1 kgCOD m-3 d-1. However, for an OLR of 14.2 kgCOD m-3 d-1, hydrogen production rate and hydrogen yield were higher in Rarab than in Rgluc (average hydrogen production rate of 3.2 and 2.0 LH2 L-1 d-1 and hydrogen yield of 1.10 and 0.75 molH2 mol-1substrate for Rarab and Rgluc, respectively). Lower hydrogen production in Rgluc was associated with higher lactate production. Denaturing gradient gel electrophoresis (DGGE) results revealed no significant difference on the bacterial community composition between operational periods and between the reactors. Increased hydrogen production was observed in batch experiments when hydrogen partial pressure was kept low, both with arabinose and glucose as substrate. Sugars were completely consumed and hydrogen production stimulated (62% higher) when pH 7 was used instead of pH 5.5. Continuous hydrogen production rate from arabinose was significantly higher than from glucose, when higher organic loading rate was used. The effect of hydrogen partial pressure on hydrogen production from glucose in batch mode was related to the extent of sugar utilization and not to the efficiency of substrate conversion to hydrogen. Furthermore, at pH 7.0, sugars uptake, hydrogen production and yield were higher than at pH 5.5, with both arabinose and glucose as substrates.
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