Transcriptome analysis reveals liver metabolism programming in kids from nutritional restricted goats during mid-gestation.
Transcriptome analysis reveals liver metabolism programming in kids from nutritional restricted goats during mid-gestation.
复制标题
转录组分析揭示了妊娠中期营养受限山羊孩子的肝脏代谢程序
作者：
Yang C;Zhou X;Yang H;Gebeyew K;Yan Q;Zhou C;He Z;Tan Z
母亲在怀孕期间的营养限制会导致代谢紊乱，威胁到人类和动物后代的健康。然而，营养不良如何影响胎儿或出生后后代肝脏代谢的分子机制仍不清楚。以山羊（Capra hircus）为实验动物，研究妊娠中期母山羊营养不良时胎肝转录组的变化，并探讨当妊娠中期母山羊营养恢复到正常水平时，这些变化是否会消失。对53只妊娠山羊进行对照（100%的维持要求，CON）或限制（妊娠第45天至第100天维持要求的60%，然后再调整，RES）饮食。从妊娠第100天的胎羊和出生后第90天的山羊幼崽中收集总共16个肝脏样品，以使用RNA-Seq获得肝脏转录谱。肝转录组的主成分分析显示，生长阶段（胎儿和幼儿）而非给药明显分离。与FC组相比，母亲营养不良使FR组胎肝中86个基因表达上调，76个基因表达下调。KEGG途径分析表明，DEG主要富集于蛋白质消化吸收、类固醇合成、碳水化合物消化吸收和胆汁分泌。在KR与KC比较中，共发现118个显著差异基因（变化倍数> 1.2，FDR < 0.1），其中79个基因表达上调，39个基因表达下调，这些差异基因主要集中在氨基酸生物合成、柠檬酸循环、缬氨酸、亮氨酸和异亮氨酸生物合成以及碳代谢中。肝脏转录组分析表明，母体营养不良促进了胎羊肝脏蛋白质的消化吸收，但抑制了重组后小山羊肝脏的碳水化合物代谢和柠檬酸循环。结果表明，妊娠中期母体营养不良在分子水平上导致了小山羊肝脏代谢程序化。
Maternal nutrient restriction during pregnancy causes a metabolic disorder that threatens the offspring’s health in humans and animals. However, the molecular mechanism of how undernutrition affecting hepatic metabolism of fetal or postnatal offspring is still unclear. We aimed to investigate transcriptomic changes of fetal livers in response to maternal malnutrition in goats during mid-gestation and to explore whether these changes would disappear when the nutrition was recovered to normal level during mid-gestation using goats (Capra hircus) as the experimental animals. Fifty-three pregnant goats were subjected to a control (100% of the maintenance requirements, CON) or a restricted (60% of the maintenance requirements on day 45 to day 100 of gestation and then realimentation, RES) diet. A total of 16 liver samples were collected from fetal goats on day 100 of gestation and goat kids of postnatal day 90 to obtain hepatic transcriptional profiles using RNA-Seq. Principal component analysis of the hepatic transcriptomes presented a clear separation by growth phase (fetus and kid) rather than treatment. Maternal undernutrition up-regulated 86 genes and down-regulated 76 genes in the fetal liver of the FR group as compared to the FC group. KEGG pathway analysis showed the DEGs mainly enriched in protein digestion and absorption, steroid biosynthesis, carbohydrate digestion and absorption and bile secretion. A total of 118 significant DEGs (fold change > 1.2 and FDR < 0.1) within KR vs. KC comparison was identified with 79 up-regulated genes and down-regulated 39 genes, and these DEGs mainly enriched in the biosynthesis of amino acids, citrate cycle, valine, leucine and isoleucine biosynthesis and carbon metabolism. Hepatic transcriptome analysis showed that maternal undernutrition promoted protein digestion and absorption in the fetal livers, while which restrained carbohydrate metabolism and citric acid cycle in the livers of kid goats after realimentation. The results indicate that maternal undernutrition during mid-gestation causes hepatic metabolism programming in kid goats on a molecular level.
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影响因子：
4.4
作者：
Keogh K;Kenny DA;Cormican P;Kelly AK;Waters SM
通讯作者：
Waters SM
DOI：
10.1016/j.cbd.2010.12.002
发表时间：
2011-06-01
影响因子：
3
作者：
Faulconnier, Y.;Chilliard, Y.;Leroux, C.
通讯作者：
Leroux, C.
影响因子：
4.8
作者：
GRUNE, T;REINHECKEL, T;DAVIES, KJA
通讯作者：
DAVIES, KJA
母羊孕期限制采食量对胎羊脐血免疫指标及肝脏Toll样受体信号通路的影响
影响因子：
7
作者：
Chen, Wenxun;Yan, Qiongxian;Tan, Zhiliang
通讯作者：
Tan, Zhiliang
影响因子：
1.9
作者：
Chadio, Stella;Kotsampasi, Basiliki;Plakokefalos, Elias
通讯作者：
Plakokefalos, Elias