The effects of secretion factors from umbilical cord derived mesenchymal stem cells on osteogenic differentiation of mesenchymal stem cells.
The effects of secretion factors from umbilical cord derived mesenchymal stem cells on osteogenic differentiation of mesenchymal stem cells.
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脐带间充质干细胞分泌因子对间充质干细胞成骨分化的影响
DOI：
10.1371/journal.pone.0120593
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Li G
中科院分区：
文献类型：
--
作者：
Wang KX;Xu LL;Rui YF;Huang S;Lin SE;Xiong JH;Li YH;Lee WY;Li G
间充质干细胞（mesenchymal stem cells，MSCs）合成的因子包括多种生长因子、细胞因子、exosomes和microRNA等，这些因子可能影响MSCs的分化能力。本研究探讨了人脐带间充质干细胞（human umbilical cord derived mesenchymal stem cells，hUCMSCs）分泌因子对人骨髓间充质干细胞（human bone marrow derived mesenchymal stem cells，hBMSCs）成骨的影响。结果显示，在无成骨诱导液（OIM）的条件下，20 μg/ml的hUCMSCs分泌因子可诱导hBMSCs向成骨细胞分化，细胞内钙存款量（茜素红染色）明显增加。真实的时间定量逆转录-聚合酶链反应（真实的时间qRT-PCR）显示，hUCMSCs分泌因子处理后，成骨相关基因ALP、BMP 2、OCN、Osterix、Col 1 α和Runx 2的表达显著上调。此外，我们发现10 μg hUCMSCs分泌因子与2×105 hBMSCs一起在HA/TCP支架中促进裸鼠异位成骨。局部应用10 μg hUCMSCs分泌因子与50 μl 2%透明质酸水凝胶和1×105大鼠骨髓间充质干细胞（rBMSCs），在4周和8周时均能显著促进大鼠颅骨临界缺损模型的骨修复。此外，接受hUCMSCs分泌因子治疗的组在缺损区域中具有比对照组更多的软骨和骨再生。综上所述，这些发现表明hUCMSCs分泌因子可以启动骨髓MSCs的成骨，促进骨修复。我们的研究表明hUCMSCs分泌因子可能是促进骨再生的潜在来源。
Factors synthesized by mesenchymal stem cells (MSCs) contain various growth factors, cytokines, exosomes and microRNAs, which may affect the differentiation abilities of MSCs. In the present study, we investigated the effects of secretion factors of human umbilical cord derived mesenchymal stem cells (hUCMSCs) on osteogenesis of human bone marrow derived MSCs (hBMSCs). The results showed that 20 μg/ml hUCMSCs secretion factors could initiate osteogenic differentiation of hBMSCs without osteogenic induction medium (OIM), and the amount of calcium deposit (stained by Alizarin Red) was significantly increased after the hUCMSCs secretion factors treatment. Real time quantitative reverse transcription-polymerase chain reaction (real time qRT-PCR) demonstrated that the expression of osteogenesis-related genes including ALP, BMP2, OCN, Osterix, Col1α and Runx2 were significantly up-regulated following hUCMSCs secretion factors treatment. In addition, we found that 10 μg hUCMSCs secretion factors together with 2×105 hBMSCs in the HA/TCP scaffolds promoted ectopic bone formation in nude mice. Local application of 10 μg hUCMSCs secretion factors with 50 μl 2% hyaluronic acid hydrogel and 1×105 rat bone marrow derived MSCs (rBMSCs) also significantly enhanced the bone repair of rat calvarial bone critical defect model at both 4 weeks and 8 weeks. Moreover, the group that received the hUCMSCs secretion factors treatment had more cartilage and bone regeneration in the defect areas than those in the control group. Taken together, these findings suggested that hUCMSCs secretion factors can initiate osteogenesis of bone marrow MSCs and promote bone repair. Our study indicates that hUCMSCs secretion factors may be potential sources for promoting bone regeneration.
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影响因子：
5.2
作者：
Baksh, Dolores;Yao, Raphael;Tuan, Rocky S.
通讯作者：
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