Traceless cross-linker for photocleavable bioconjugation.
Traceless cross-linker for photocleavable bioconjugation.
复制标题
可获光生物缀合的无纹状体交联。
DOI：
10.1021/bc200343u
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发表时间：
2012-04-18
影响因子：
4.7
通讯作者：
Chen, Liaohai
中科院分区：
文献类型：
--
作者：
Wang, Rong;Yan, Funing;Qiu, Dengli;Jeong, Jae-Sun;Jin, Qiaoling;Kim, Tae-Young;Chen, Liaohai
光响应性生物缀合使得开发用于药物发现、疾病诊断和高通量筛选等的新方法成为可能。本文报道了一种无痕光致交联剂--二6-（3-琥珀酰亚胺羰氧基甲基-4-硝基苯氧基）己酸二硫醚二乙醇酯（SCNE）的性质。证实了这种光可裂解交联剂的无痕性和生物相容性。因此，我们展示了其在可逆噬菌体颗粒固定中的应用，可以为直接的单噬菌体筛选提供平台。我们还将其应用于蛋白质打印，其中SCNE充当“光擦除器”，以简单，清洁和光可控的方式去除所需区域的交联蛋白质分子。我们进一步展示了两层原子力显微镜（AFM）的方法，使用SCNE进行两个后续的AFM原位任务。该方法允许在同一局部区域引导蛋白质递送和随后的高分辨率成像，从而开辟了监测活细胞中蛋白质功能的可能性。结果表明，SCNE是一种通用的交联剂，可用于广泛的应用，其中光裂解确保清洁和远程可控的释放生物分子从基板。
Photo-responsive bioconjugation empowers the development of novel methods for drug discovery, disease diagnosis, and high-throughput screening, among the others. In this paper, we report on the characteristics of a traceless photo-cleavable cross-linker, di 6-(3-succinimidyl carbonyloxymethyl-4-nitro-phenoxy)-hexanoic acid disulfide diethanol ester (SCNE). The traceless feature and the biocompatibility of this photo-cleavable cross-linking reagent were corroborated. Consequently, we demonstrated its application in reversible phage particle immobilization that could provide a platform for direct single phage screening. We also applied it in protein-photoprinting, where SCNE acts as a “photo-eraser” to remove the cross-linked protein molecules at a desired region in a simple, clean and light-controllable fashion. We further demonstrated the two-tier atomic force microscopic (AFM) method that uses SCNE to carry out two subsequent AFM tasks in situ. The approach allows guided protein delivery and subsequent high-resolution imaging at the same local area, thus opens up the possibility of monitoring protein functions in live cells. The results imply that SCNE is a versatile cross-linker that can be used for a wide range of applications where photo-cleavage ensures clean and remote-controllable release of biological molecules from a substrate.
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DOI：
10.1007/10_2006_036
发表时间：
2007-01-01
期刊：
WHITE BIOTECHNOLOGY
影响因子：
--
作者：
Becker, Thomas;Hitzmann, Bernd;Ulber, Roland
通讯作者：
Ulber, Roland
影响因子：
4.7
作者：
Kaneda, M;Sadakane, Y;Hatanaka, Y
通讯作者：
Hatanaka, Y
影响因子：
64.8
作者：
Finch, EA;Augustine, GJ
通讯作者：
Augustine, GJ
影响因子：
--
作者：
Chaudhary, A;Chen, J;Prestwich, GD
通讯作者：
Prestwich, GD
影响因子：
56.9
作者：
Lee, KB;Park, SJ;Mrksich, M
通讯作者：
Mrksich, M