Effects of sex and aging on the immune cell landscape as assessed by single-cell transcriptomic analysis.
Effects of sex and aging on the immune cell landscape as assessed by single-cell transcriptomic analysis.
复制标题
DOI：
10.1073/pnas.2023216118
复制
发表时间：
2021-08-17
影响因子：
11.1
通讯作者：
Su W
中科院分区：
文献类型：
--
作者：
Huang Z;Chen B;Liu X;Li H;Xie L;Gao Y;Duan R;Li Z;Zhang J;Zheng Y;Su W
免疫功能的差异源于多种因素，包括性别和年龄。然而，这些变量在免疫中的具体作用仍然难以捉摸。我们在单细胞分辨率下分析了年轻和老年男性和女性的免疫细胞，并构建了血液循环免疫细胞的精确图谱。年轻女性的T细胞和B细胞激活信号高于男性，而衰老增加了免疫细胞、细胞组成和炎症信号的性别相关差异。此外，男性在衰老过程中表现出更高的炎症因子积累，而细胞间通讯分析显示，随着年龄的增长，女性和男性之间的基因表达趋势不同。这些发现可能有助于理解整个生命周期中免疫力和疾病易感性性别差异的机制。性别和衰老影响人类免疫系统，导致对感染、自身免疫和癌症的不同反应。然而，性别和衰老对免疫系统的影响尚未完全阐明。使用小的条件RNA测序，我们发现，与男性相比，女性外周血中自然杀伤（NK）细胞的百分比较低，浆细胞的百分比较高。生物信息学显示，年轻女性表现出与T和B细胞活化相关的途径的过度表达。此外，细胞间通讯分析揭示了女性BAFF/APRIL系统活性增加的证据。值得注意的是，衰老增加了单核细胞的百分比，降低了血液中幼稚T细胞的百分比和性别之间差异表达基因的数量。老年男性表达更高水平的炎症基因。总的来说，结果表明，女性在循环中有更多的浆细胞和更强的BAFF/APRIL系统，这与更强的适应性免疫反应一致。相比之下，男性血液中NK细胞的百分比更高，某些促炎基因的表达更高。总的来说，这项工作扩展了我们对人类免疫系统性别差异的认识。
Differences in immune functioning stem from multiple factors, including sex and aging. However, the specific roles of these variables in immunity remain elusive. We profiled immunocytes from young and old males and females at single-cell resolution and constructed a precise atlas of blood-circulating immunocytes. T cell– and B cell–activated signals were higher in young females than males, while aging increased the sex-related differences in immunocytes, cellular composition, and inflammatory signaling. Additionally, males showed a higher accumulation of inflammatory factors during aging, whereas cell–cell communication analysis revealed different trends in gene expression between females and males with aging. These findings might aid in the understanding of the mechanisms underlying sex-based differences in immunity and disease susceptibility across the lifespan. Sex and aging influence the human immune system, resulting in disparate responses to infection, autoimmunity, and cancer. However, the impact of sex and aging on the immune system is not yet fully elucidated. Using small conditional RNA sequencing, we found that females had a lower percentage of natural killer (NK) cells and a higher percentage of plasma cells in peripheral blood compared with males. Bioinformatics revealed that young females exhibited an overrepresentation of pathways that relate to T and B cell activation. Moreover, cell–cell communication analysis revealed evidence of increased activity of the BAFF/APRIL systems in females. Notably, aging increased the percentage of monocytes and reduced the percentage of naïve T cells in the blood and the number of differentially expressed genes between the sexes. Aged males expressed higher levels of inflammatory genes. Collectively, the results suggest that females have more plasma cells in the circulation and a stronger BAFF/APRIL system, which is consistent with a stronger adaptive immune response. In contrast, males have a higher percentage of NK cells in blood and a higher expression of certain proinflammatory genes. Overall, this work expands our knowledge of sex differences in the immune system in humans.
登录
查看更多内容
癌症死亡率和生存中的性别差异。
DOI：
10.1158/1055-9965.epi-11-0246
发表时间：
2011-08
期刊：
Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology
影响因子：
--
作者：
Cook MB;McGlynn KA;Devesa SS;Freedman ND;Anderson WF
通讯作者：
Anderson WF
影响因子：
7.8
作者：
Giefing-Kröll C;Berger P;Lepperdinger G;Grubeck-Loebenstein B
通讯作者：
Grubeck-Loebenstein B
影响因子：
5.6
作者：
Hao, Sha;Zhao, Junli;Hou, Yayi
通讯作者：
Hou, Yayi
影响因子：
9.1
作者：
Jonsson, MV;Szodoray, P;Skarstein, K
通讯作者：
Skarstein, K
影响因子：
5.5
作者：
McCarthy, Eoghan M.;Lee, Ruth Z.;Jefferies, Caroline A.
通讯作者：
Jefferies, Caroline A.