Development of a High-Density Genetic Map Based on Specific Length Amplified Fragment Sequencing and Its Application in Quantitative Trait Loci Analysis for Yield-Related Traits in Cultivated Peanut.
Development of a High-Density Genetic Map Based on Specific Length Amplified Fragment Sequencing and Its Application in Quantitative Trait Loci Analysis for Yield-Related Traits in Cultivated Peanut.
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基于特定长度扩增片段测序的高密度遗传图谱构建及其在栽培花生产量相关性状数量性状位点分析中的应用
DOI：
10.3389/fpls.2018.00827
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发表时间：
2018
影响因子：
5.6
通讯作者：
Liao B
中科院分区：
文献类型：
--
作者：
Wang Z;Huai D;Zhang Z;Cheng K;Kang Y;Wan L;Yan L;Jiang H;Lei Y;Liao B
高密度遗传图谱（HDGM）是基因组研究和数量性状基因座（QTL）定位的重要工具。然而，花生DNA多态性频率低，限制了可用标记的数量，阻碍了HDGM的构建。本研究利用特异位点扩增片段测序（SLAF-seq）技术构建了一张高质量SNPs数量最多的花生遗传图谱，并构建了一个新的RIL群体（“ZH 16”דsd-H1”）。构建的HDGM包含3，630个SNP标记，分属于20个连锁群（LGs）上的2，636个bin，长度为2，098.14 cM，平均标记距离为0.58 cM。该HDGM用于以下共线比较、支架锚定和基因组表征的分析，包括花生中的重组率和分离畸变。对14个产量相关性状进行QTL定位，共检测到62个QTL，分布在12条染色体上，且在3个环境中均存在QTL共定位现象。在B 06和B 07的染色体末端分别检测到两个稳定的粒荚相关性状QTL。本研究的HDGM基因的构建和产量相关性状的QTL分析为基因的精细定位和功能分析以及分子标记辅助育种提供了有用的信息。
High-density genetic maps (HDGMs) are very useful for genomic studies and quantitative trait loci (QTL) mapping. However, the low frequency of DNA polymorphisms in peanut has limited the quantity of available markers and hindered the construction of a HDGM. This study generated a peanut genetic map with the highest number of high-quality SNPs based on specific locus amplified fragment sequencing (SLAF-seq) technology and a newly constructed RIL population (“ZH16” × “sd-H1”). The constructed HDGM included 3,630 SNP markers belonging to 2,636 bins on 20 linkage groups (LGs), and it covers 2,098.14 cM in length, with an average marker distance of 0.58 cM. This HDGM was applied for the following collinear comparison, scaffold anchoring and analysis of genomic characterization including recombination rates and segregation distortion in peanut. For QTL mapping of investigated 14 yield-related traits, a total of 62 QTLs were detected on 12 chromosomes across 3 environments, and the co-localization of QTLs was observed for these traits which were significantly correlated on phenotype. Two stable co-located QTLs for seed- and pod-related traits were significantly identified in the chromosomal end of B06 and B07, respectively. The construction of HDGM and QTL analysis for yield-related traits in this study provide useful information for fine mapping and functional analysis of genes as well as molecular marker-assisted breeding.
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影响因子：
5.3
作者：
Hong Y;Chen X;Liang X;Liu H;Zhou G;Li S;Wen S;Holbrook CC;Guo B
通讯作者：
Guo B
栽培花生 (Arachishypogaea L.) 荚果和仁相关性状的数量性状位点分析
影响因子：
2.9
作者：
Chen W;Jiao Y;Cheng L;Huang L;Liao B;Tang M;Ren X;Zhou X;Chen Y;Jiang H
通讯作者：
Jiang H
利用特定长度扩增片段测序构建葡萄（Vitis vinifera L. x Vitis amurensis Rupr.）高密度遗传图谱
影响因子：
5.6
作者：
Guo Y;Shi G;Liu Z;Zhao Y;Yang X;Zhu J;Li K;Guo X
通讯作者：
Guo X
DOI：
10.1534/g3.113.007617
发表时间：
2014-01-10
期刊：
G3 (Bethesda, Md.)
影响因子：
--
作者：
Bertioli DJ;Ozias-Akins P;Chu Y;Dantas KM;Santos SP;Gouvea E;Guimarães PM;Leal-Bertioli SC;Knapp SJ;Moretzsohn MC
通讯作者：
Moretzsohn MC
影响因子：
1.5
作者：
Belamkar, Vikas;Selvaraj, Michael Gomez;Burow, Mark D.
通讯作者：
Burow, Mark D.