Purification and Characterization of Biofilm-Associated EPS Exopolysaccharides from ESKAPE Organisms and Other Pathogens.
Purification and Characterization of Biofilm-Associated EPS Exopolysaccharides from ESKAPE Organisms and Other Pathogens.
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DOI：
10.1371/journal.pone.0067950
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Nelson DC
中科院分区：
文献类型：
--
作者：
Bales PM;Renke EM;May SL;Shen Y;Nelson DC
在细菌生物膜中，高分子量分泌的胞外多糖可以充当支架，额外的碳水化合物、蛋白质、脂质和核酸粘附于其上，形成发育中的生物膜的基质。在这里，我们报告的方法，提取和纯化高分子量（>15 kDa）胞外多糖的生物膜的8个人类病原体，包括葡萄球菌属，克雷伯氏菌，不动杆菌属，假单胞菌属，和大肠杆菌O 157：H7的产酶菌株。糖基组成分析表明，在所有菌株中，铜绿假单胞菌和A.鲍曼不动杆菌胞外多糖中甘露糖含量为80-90%;肺炎链球菌和S. epidermidis菌株和E.大肠杆菌，甘露糖浓度为10%左右。半乳糖和葡萄糖也存在于所有八种菌株中，通常作为第二和第三最丰富的碳水化合物。在8株菌中的6株中发现了N-乙酰氨基葡萄糖和半乳糖醛酸，而在8株菌中的5株中发现了阿拉伯糖、岩藻糖、鼠李糖和木糖。对于连接分析，检测到33种不同的残基-连接组合，其中最丰富的是甘露糖连接的部分，与组成分析一致。分析的两种铜绿假单胞菌菌株的胞外多糖与Psl碳水化合物一致，但不与Pel或藻酸盐一致。色葡萄表皮葡萄球菌菌株具有富含甘露糖和葡萄糖的组成，这分别与先前描述的粘液相关抗原（SAA）和细胞外粘液物质（ESS）一致，但未检测到多糖细胞内粘附（PIA）。E. coli，K.肺炎杆菌和A.基于碳水化合物的组成和/或比例分析，鲍曼不动杆菌似乎是新的。
In bacterial biofilms, high molecular weight, secreted exopolysaccharides can serve as a scaffold to which additional carbohydrates, proteins, lipids, and nucleic acids adhere, forming the matrix of the developing biofilm. Here we report methods to extract and purify high molecular weight (>15 kDa) exopolysaccharides from biofilms of eight human pathogens, including species of Staphylcococcus, Klebsiella, Acinetobacter, Pseudomonas, and a toxigenic strain of Escherichia coli O157:H7. Glycosyl composition analysis indicated a high total mannose content across all strains with P. aeruginosa and A. baumannii exopolysaccharides comprised of 80–90% mannose, K. pneumoniae and S. epidermidis strains containing 40–50% mannose, and E. coli with ∼10% mannose. Galactose and glucose were also present in all eight strains, usually as the second and third most abundant carbohydrates. N-acetyl-glucosamine and galacturonic acid were found in 6 of 8 strains, while arabinose, fucose, rhamnose, and xylose were found in 5 of 8 strains. For linkage analysis, 33 distinct residue-linkage combinations were detected with the most abundant being mannose-linked moieties, in line with the composition analysis. The exopolysaccharides of two P. aeruginosa strains analyzed were consistent with the Psl carbohydrate, but not Pel or alginate. The S. epidermidis strain had a composition rich in mannose and glucose, which is consistent with the previously described slime associated antigen (SAA) and the extracellular slime substance (ESS), respectively, but no polysaccharide intracellular adhesion (PIA) was detected. The high molecular weight exopolysaccharides from E. coli, K. pneumoniae, and A. baumannii appear to be novel, based on composition and/or ratio analysis of carbohydrates.
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影响因子：
3.1
作者：
Cramton, SE;Gerke, C;Götz, F
通讯作者：
Götz, F
影响因子：
4.9
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Kaplan, JB;Ragunath, C;Ramasubbu, N
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Ramasubbu, N
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3.1
作者：
ELSASSERBEILE, U;FRIEBOLIN, H;STIRM, S
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STIRM, S
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作者：
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作者：
Boucher, Helen W.;Talbot, George H.;Bartlett, John
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