Characterizing Physical Interactions between Male and Female Mosquitoes (Aedes aegypti) in Relation to Female Receptivity and Insemination Outcomes Using a Hydrophobic Fluorescent Dye.
Characterizing Physical Interactions between Male and Female Mosquitoes (Aedes aegypti) in Relation to Female Receptivity and Insemination Outcomes Using a Hydrophobic Fluorescent Dye.
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DOI：
10.1093/icb/icad041
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发表时间：
2023-08-23
影响因子：
2.6
通讯作者：
中科院分区：
文献类型：
--
作者：
埃及伊蚊，即黄热病蚊子，作为致病病原体的媒介，对全球人类健康构成重大威胁。这个物种的雌性通常只交配一次。从这一次交配事件中，雌性储存了足够的精子来为她一生中产生的多窝卵受精。交配会引起雌性行为和生理上的巨大变化，包括终生抑制交配的接受能力。雌性排斥行为包括雄性回避、腹部扭转、甩翅、踢腿、不打开阴道板或不挤出产卵器。许多这些事件发生的规模太小或太快，肉眼无法看到，所以高分辨率的摄像技术被用来观察这些行为。然而，摄像可能是劳动密集型的，需要专门的设备，并且经常需要约束动物。我们使用了一种高效、低成本的方法来记录雄性和雌性在尝试交配和成功交配期间的身体接触，通过记录解剖后的精囊填充来确定。疏水性油基荧光染料可应用于一只动物的腹部尖端，并可随后在生殖器接触时转移到异性动物的生殖器上。我们的数据表明，雄性蚊子与受精卵和未受精卵的雌性蚊子都有高水平的接触，而且雄性蚊子试图与更多的雌性蚊子交配，而不是成功受精。被破坏的雌蚊与多个雄蚊交配并产生后代，将染料传递给每只雄蚊。这些数据表明，身体上的交配相互作用独立于雌性对配偶的接受性，许多这些相互作用代表了不成功的交配尝试，而不是导致受精。
Aedes aegypti, the yellow fever mosquito, presents a major threat to human health across the globe as a vector of disease-causing pathogens. Females of this species generally mate only once. From this single mating event, the female stores sufficient sperm to fertilize the multiple clutches of eggs produced during her lifetime. Mating causes dramatic changes in the female’s behavior and physiology, including a lifetime suppression of her mating receptivity. Female rejection behaviors include male avoidance, abdominal twisting, wing-flicking, kicking, and not opening vaginal plates or extruding the ovipositor. Many of these events occur on a scale that is too miniscule or fast to see by eye, so high-resolution videography has been used to observe these behaviors instead. However, videography can be labor intensive, require specialized equipment, and often requires restrained animals. We used an efficient, low-cost method to record physical contact between males and females during attempted and successful mating, determined by recording spermathecal filling after dissection. A hydrophobic oil-based fluorescent dye can be applied to the abdominal tip of one animal and can be subsequently transferred to the genitalia of animals of the opposite sex when genital contact occurs. Our data indicate that male mosquitoes make high levels of contact with both receptive and unreceptive females and that males attempt to mate with more females than they successfully inseminate. Female mosquitoes with disrupted remating suppression mate with and produce offspring from multiple males, transferring dye to each. These data suggest that physical copulatory interactions occur independently of the female’s receptivity to mate and that many of these interactions represent unsuccessful mating attempts that do not result in insemination.
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影响因子：
3.7
作者：
Carvalho DO;Chuffi S;Ioshino RS;Marques ICS;Fini R;Costa MK;Araújo HRC;Costa-da-Silva AL;Kojin BB;Capurro ML
通讯作者：
Capurro ML
影响因子：
--
作者：
Rogers, D. J.;Wilson, A. J.;Hay, S. I.;Graham, A. J.
通讯作者：
Graham, A. J.
影响因子：
2.5
作者：
Cator, Lauren J.;Harrington, Laura C.
通讯作者：
Harrington, Laura C.
影响因子：
2.2
作者：
HISS, EA;FUCHS, MS
通讯作者：
FUCHS, MS
DOI：
10.4269/ajtmh.2012.11-0225
发表时间：
2012-04-01
影响因子：
3.3
作者：
Helinski, Michelle E. H.;Valerio, Laura;Harrington, Laura C.
通讯作者：
Harrington, Laura C.