Mosquito pornoscopy: Observation and interruption of Aedes aegypti copulation to determine female polyandric event and mixed progeny.
Mosquito pornoscopy: Observation and interruption of Aedes aegypti copulation to determine female polyandric event and mixed progeny.
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DOI：
10.1371/journal.pone.0193164
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发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
Capurro ML
中科院分区：
文献类型：
--
作者：
Carvalho DO;Chuffi S;Ioshino RS;Marques ICS;Fini R;Costa MK;Araújo HRC;Costa-da-Silva AL;Kojin BB;Capurro ML
埃及伊蚊是世界上最重要的虫媒病毒媒介，目前正在评估新的战略。生物学研究提到埃及伊蚊出现第二配偶可能会干扰媒介控制规划，其中涉及雄性蚊子释放技术。本研究提出了不同的实验来证明混合后代的发生。混合雄性杂交（使用不同类型的雄性与处女雌性的组合）显示没有一妻多夫制的雌性。在每个淋养循环中，雄性代入的个体杂交也没有出现雌性多雄现象。个体杂交间隔20分钟，随着雄代的变化，只有少数雌代出现杂交后代。在三个不同的时刻，A组的交媾时间完全，B组的交媾在开始后5-7秒中断；C组，开始交配3秒后中断交配。综上所述，A组以首雄的独特后代居多；B组杂交后代的频率较高，而C组杂交后代大部分属于第二雄。综上所述，只有当交配中断时，才有可能出现一个可行的第二配偶和混合后代；否则，大副负责混合后代。
Ades aegypti is the most important arbovirus vector in the world, and new strategies are under evaluation. Biological studies mentioning the occurrence of a second mate in Aedes aegypti can interfere with vector control program planning, which involves male mosquito release technique. This study presents different experiments to show the occurrence of mixed progeny. Mixed male crosses (using a combination of different type of males in confinement with virgin females) showed no polyandric female. Individual crosses with male substitution in every gonotrophic cycle also did not show any polyandric female. Individual crosses with a 20 minutes interval, with subsequent male change, showed that only a few females presented mixed offspring. The copulation breach in three different moments, group A with full coitus length, group B the coitus was interrupted in 5–7 seconds after the start; and group C, which the copulation was interrupted 3 seconds after started. In summary, group A showed a majority of unique progeny from the first male; group B showed the higher frequency of mixed offspring and group C with the majority of the crosses belonging to the second male. To conclude, the occurrence of a viable second mate and mixed offspring is only possible when the copulation is interrupted; otherwise, the first mate is responsible for mixed progeny.
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影响因子：
2.2
作者：
HISS, EA;FUCHS, MS
通讯作者：
FUCHS, MS
影响因子：
3.7
作者：
Boyer S;Toty C;Jacquet M;Lempérière G;Fontenille D
通讯作者：
Fontenille D
影响因子：
3
作者：
Helinski ME;Hassan MM;El-Motasim WM;Malcolm CA;Knols BG;El-Sayed B
通讯作者：
El-Sayed B
转基因蚊子埃及埃及雄性的适应性，载有主要的致命遗传系统。
影响因子：
3.7
作者：
Massonnet-Bruneel B;Corre-Catelin N;Lacroix R;Lees RS;Hoang KP;Nimmo D;Alphey L;Reiter P
通讯作者：
Reiter P
DOI：
10.3791/3579
发表时间：
2014-01-04
期刊：
Journal of visualized experiments : JoVE
影响因子：
--
作者：
Carvalho DO;Nimmo D;Naish N;McKemey AR;Gray P;Wilke AB;Marrelli MT;Virginio JF;Alphey L;Capurro ML
通讯作者：
Capurro ML