Microbial uptake dynamics of choline and glycine betaine in coastal seawater
Microbial uptake dynamics of choline and glycine betaine in coastal seawater
复制标题
沿海海水中胆碱和甘氨酸甜菜碱的微生物吸收动态
DOI：
10.1002/lno.12056
复制
发表时间：
2022
影响因子：
4.5
通讯作者：
Mausz M
中科院分区：
文献类型：
--
作者：
Mausz M
胆碱和甘氨酸甜菜碱(GBT)被用作渗透压物质来抵消渗透胁迫，但也是许多海洋微生物的重要营养来源。这些底物的细菌分解代谢会导致甲胺和甲烷等气候活性微量气体的产生。利用放射性示踪剂，我们研究了原核生物对胆碱/GBT的摄取，并确定了推动这些过程的生物和非生物因素。动力学摄取参数显示对两种渗透分子的高亲和力(NM范围)，并显示出胆碱摄取的季节性模式。摄取参数的广义线性模型表明，海洋表面温度和盐度对原核生物对这两种渗透分子的摄取有显著影响。硅藻的存在显著影响原核生物胆碱/GBT的摄取动力学。胆碱的摄取与棕囊藻和甲藻的发生有进一步的关系，棕囊藻春季在浮游植物群落中高度丰富，而甲藻在夏季大量出现。虽然红杆菌科是原核生物摄取胆碱的最重要的细菌驱动因素，但原核生物摄取GBT与不同的类群有关，如SAR11(膜细菌目)和GammaProtebacteria，这表明GBT分解代谢的能力比以前认为的更大。此外，使用一种新开发的方法，我们确定了海水中溶解的GBT浓度的第一个可用数据，并发现这两个渗透分子都处于亚纳米分子范围。总之，这项研究提高了我们对这些对环境重要的渗透分子的生物地球化学循环的理解，并强调了它们的循环可能会如何受到气候变化的影响。
Choline and glycine betaine (GBT) are utilized as osmolytes to counteract osmotic stress, but also constitute important nutrient sources for many marine microbes. Bacterial catabolism of these substrates can then lead to the production of climate active trace gases such as methylamine and methane. Using radiotracers, we investigated prokaryotic choline/GBT uptake and determined biotic and abiotic factors driving these processes in the Western English Channel, UK. Kinetic uptake parameters indicated high affinity (nM range) for both osmolytes and showed a seasonal pattern for choline uptake. Generalized linear modeling of uptake parameters suggested a significant influence of sea surface temperature and salinity on prokaryotic uptake of both osmolytes. The presence of diatoms significantly influenced prokaryotic choline/GBT uptake dynamics. Choline uptake was further related to the occurrence ofPhaeocystisspp., which were highly abundant in the phytoplankton community during spring, and dinoflagellates abundance during summer. While Rhodobacteraceae were the most important bacterial drivers for prokaryotic choline uptake, prokaryotic GBT uptake was associated with various groups such as SAR11 (Pelagibacterales) and Gammaproteobacteria, suggesting a wider capacity for GBT catabolism than previously recognized. Furthermore, using a newly developed approach we determined the first available data for dissolved GBT concentrations in seawater and found both osmolytes to be at the sub‐nanomolar range. Together, this study improves our understanding of the biogeochemical cycling of these environmentally important osmolytes and highlights how their cycles may be affected by a changing climate.
登录
查看更多内容
影响因子：
5.2
作者：
Zecher K;Hayes KR;Philipp B
通讯作者：
Philipp B
河口水域细菌对胆碱的吸收及其向甘氨酸甜菜碱的转化
影响因子：
4.4
作者：
R. Kiene
通讯作者：
R. Kiene
DOI：
--
发表时间：
1990
期刊：
影响因子：
--
作者：
M. Roulier;B. Palenik;F. Morel
通讯作者：
F. Morel
影响因子：
2.4
作者：
Keller, MD;Kiene, RP;Bellows, WK
通讯作者：
Bellows, WK
影响因子：
6.2
作者：
Beale, Rachael;Airs, Ruth
通讯作者：
Airs, Ruth