The over-expression of an Arabidopsis B3 transcription factor, ABS2/NGAL1, leads to the loss of flower petals.
The over-expression of an Arabidopsis B3 transcription factor, ABS2/NGAL1, leads to the loss of flower petals.
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拟南芥 B3 转录因子 ABS2/NGAL1 的过度表达导致花瓣损失
DOI：
10.1371/journal.pone.0049861
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Yu F
中科院分区：
文献类型：
--
作者：
Shao J;Liu X;Wang R;Zhang G;Yu F
转录调控涉及植物发育的许多方面，主要通过转录因子（TF）的作用来实现。为了研究植物发育的机制，我们对具有异常芽发育的突变体进行了遗传筛选。采用激活标签的方法，我们分离到一个功能获得性突变体abs 2 -1D（异常芽2-1D）。abs 2 -1D在营养和生殖发育阶段均表现出多效性生长缺陷。我们克隆了ABS 2，它编码一个RAV亚家族的植物B3类转录因子。系统发育分析表明，ABS 2与参与花发育的NGATHA（NGA）基因密切相关，以前被命名为NGATHA-Like 1（NGAL 1）。NGAL 1在花组织中主要表达于雄蕊的根和花丝中，亚细胞定位分析表明NGAL 1在细胞核中积累。有趣的是，由组成型35 S启动子驱动的NGAL 1的过表达导致转基因植物具有明显的花缺陷，特别是花瓣缺失表型。一个功能丧失的ngal 1 -1突变体没有表现出明显的表型，这表明存在冗余的活动，也是功能获得性遗传筛选的效用。我们的研究结果表明，NGAL 1的过表达能够改变花瓣发育，以及芽发育。
Transcriptional regulations are involved in many aspects of plant development and are mainly achieved through the actions of transcription factors (TF). To investigate the mechanisms of plant development, we carried out genetic screens for mutants with abnormal shoot development. Taking an activation tagging approach, we isolated a gain-of-function mutant abs2-1D (abnormal shoot 2-1D). abs2-1D showed pleiotropic growth defects at both the vegetative and reproductive developmental stages. We cloned ABS2 and it encodes a RAV sub-family of plant B3 type of transcriptional factors. Phylogenetic analysis showed that ABS2 was closely related to NGATHA (NGA) genes that are involved in flower development and was previously named NGATHA-Like 1 (NGAL1). NGAL1 was expressed mainly in the root and the filament of the stamen in flower tissues and sub-cellular localization assay revealed that NGAL1 accumulated in the nucleus. Interestingly, over-expression of NGAL1 driven by the constitutive 35S promoter led to transgenic plants with conspicuous flower defects, particularly a loss-of-petal phenotype. A loss-of-function ngal1-1 mutant did not show obvious phenotype, suggesting the existence of redundant activities and also the utility of gain-of-function genetic screens. Our results show that the over-expression of NGAL1 is capable of altering flower petal development, as well as shoot development.
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影响因子：
64.5
作者：
MIZUKAMI, Y;MA, H
通讯作者：
MA, H
影响因子：
4.6
作者：
Brewer, PB;Howles, PA;Smyth, DR
通讯作者：
Smyth, DR
影响因子：
44.1
作者：
Hu, YX;Wang, YH;Li, JY
通讯作者：
Li, JY
影响因子：
4.6
作者：
Takeda, S;Matsumoto, N;Okada, K
通讯作者：
Okada, K
影响因子：
7.2
作者：
Krizek, BA;Lewis, MW;Fletcher, JC
通讯作者：
Fletcher, JC