Circular RNA Plek promotes fibrogenic activation by regulating the miR-135b-5p/TGF-βR1 axis after spinal cord injury.
Circular RNA Plek promotes fibrogenic activation by regulating the miR-135b-5p/TGF-βR1 axis after spinal cord injury.
复制标题
脊髓损伤后，圆形 RNA Plek 通过调节 miR-135b-5p/TGF-βR1 轴促进纤维化激活。
DOI：
10.18632/aging.203002
复制
发表时间：
2021-05-11
期刊：
影响因子：
--
通讯作者：
Wei J
中科院分区：
文献类型：
--
作者：
Wang W;He D;Chen J;Zhang Z;Wang S;Jiang Y;Wei J
Objectives: The spinal cord rarely repairs itself when damaged; however, methods for encouraging nerves to regrow are on the horizon. Although circular RNAs (circRNAs) contribute to various biological processes, including neuronal processes, their functions in the subacute phase of spinal cord injury (SCI) have not been elucidated. In this study, we identified a novel circRNA, named CircPlek, with increased expression in spinal tissues after SCI. Materials and Methods: We predicted a regulatory relationship between CircPlek and miR-135b-5p, which showed the most obvious decrease in post-SCI expression. We established the CircPlek/miR-135b-5p/transforming growth factor-beta receptor type I (TGF-βR1) axis using a bioinformatics approach and further evaluated the potential function of the interaction network in vitro. Results: We confirmed that in TGF-β1-induced fibroblasts, the overexpression of miR-135b-5p or/and inhibition of CircPlek inhibited fibrosis activation via the Smad pathway. Inhibitors of miR-135b-5p had antagonistic effects on CircPlek. Conclusions: the CircPlek/miR-135b-5p/TGF-βR1 axis may exert important functions in SCI and is a potential therapeutic target.
登录
查看更多内容
影响因子：
64.5
作者：
Kleaveland B;Shi CY;Stefano J;Bartel DP
通讯作者：
Bartel DP
影响因子：
64.5
作者：
Dias DO;Kim H;Holl D;Werne Solnestam B;Lundeberg J;Carlén M;Göritz C;Frisén J
通讯作者：
Frisén J
影响因子：
14.9
作者：
Kanehisa M;Araki M;Goto S;Hattori M;Hirakawa M;Itoh M;Katayama T;Kawashima S;Okuda S;Tokimatsu T;Yamanishi Y
通讯作者：
Yamanishi Y
影响因子：
50.3
作者：
Valeri N;Braconi C;Gasparini P;Murgia C;Lampis A;Paulus-Hock V;Hart JR;Ueno L;Grivennikov SI;Lovat F;Paone A;Cascione L;Sumani KM;Veronese A;Fabbri M;Carasi S;Alder H;Lanza G;Gafa' R;Moyer MP;Ridgway RA;Cordero J;Nuovo GJ;Frankel WL;Rugge M;Fassan M;Groden J;Vogt PK;Karin M;Sansom OJ;Croce CM
通讯作者：
Croce CM
影响因子：
4.2
作者：
Hawasli, Ammar H.;Rutlin, Jerrel;Ray, Wilson Z.
通讯作者：
Ray, Wilson Z.