The ingenious structure of central rotor apparatus in VoV1; key for both complex disassembly and energy coupling between V1 and Vo.
The ingenious structure of central rotor apparatus in VoV1; key for both complex disassembly and energy coupling between V1 and Vo.
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VOV1中中央转子设备的巧妙结构； V1和VO之间的复杂拆卸和能量耦合的关键。
DOI：
10.1371/journal.pone.0119602
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Yokoyama K
中科院分区：
文献类型：
--
作者：
Nakanishi A;Kishikawa J;Tamakoshi M;Yokoyama K
单极型旋转H+-ATP酶（VoV 1）通过由V1-DF转子轴、插座状Vo-C（真核Vo-d）和疏水转子环组成的中心转子装置的旋转，将V1的ATP合成/水解与Vo的质子转运偶联。使用亚复合物的重构实验揭示了V1-DF对Vo-C的弱结合亲和力，尽管扭矩需要在V1-DF和Vo-C之间传递以使V1和Vo之间的紧密能量耦合。V1-DF尖端的短螺旋突变引起VoV 1的分子内解偶联，这表明V1-D的短螺旋适当地装配到Vo-C的插座中是V1和Vo之间的紧密能量耦合所必需的。为了解释V1-DF和Vo-C之间相互作用的明显矛盾的性质（弱结合亲和力但对扭矩传递的严格要求），我们提出了一个模型，其中V1-DF和Vo-C之间的关系对应于一字螺丝刀和一字螺丝钉的头部之间的关系。该模型与我们先前的结果一致，其中中心转子装置不是V1与Vo结合的主要因素（Kishikawa和Yokoyama，J Biol Chem.2012 24597-24603）。
Vacuolar type rotary H+-ATPases (VoV1) couple ATP synthesis/hydrolysis by V1 with proton translocation by Vo via rotation of a central rotor apparatus composed of the V1-DF rotor shaft, a socket-like Vo-C (eukaryotic Vo-d) and the hydrophobic rotor ring. Reconstitution experiments using subcomplexes revealed a weak binding affinity of V1-DF to Vo-C despite the fact that torque needs to be transmitted between V1-DF and Vo-C for the tight energy coupling between V1 and Vo. Mutation of a short helix at the tip of V1-DF caused intramolecular uncoupling of VoV1, suggesting that proper fitting of the short helix of V1-D into the socket of Vo-C is required for tight energy coupling between V1 and Vo. To account for the apparently contradictory properties of the interaction between V1-DF and Vo-C (weak binding affinity but strict requirement for torque transmission), we propose a model in which the relationship between V1-DF and Vo-C corresponds to that between a slotted screwdriver and a head of slotted screw. This model is consistent with our previous result in which the central rotor apparatus is not the major factor for the association of V1 with Vo (Kishikawa and Yokoyama, J Biol Chem. 2012 24597-24603).
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影响因子：
16.6
作者：
通讯作者：
--
影响因子：
3.7
作者：
Kishikawa J;Ibuki T;Nakamura S;Nakanishi A;Minamino T;Miyata T;Namba K;Konno H;Ueno H;Imada K;Yokoyama K
通讯作者：
Yokoyama K
影响因子：
5.3
作者：
Parra, KJ;Kane, PM
通讯作者：
Kane, PM
影响因子：
3.2
作者：
Tamakoshi, M;Uchida, M;Oshima, T
通讯作者：
Oshima, T
影响因子：
3
作者：
Yokoyama, K;Imamura, H
通讯作者：
Imamura, H