Transcriptome expression profiling in response to drought stress in Paulownia australis.
Transcriptome expression profiling in response to drought stress in Paulownia australis.
复制标题
泡桐响应干旱胁迫的转录组表达谱分析
DOI：
10.3390/ijms15034583
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发表时间：
2014-03-17
影响因子：
5.6
通讯作者：
Deng M
中科院分区：
文献类型：
--
作者：
Dong Y;Fan G;Zhao Z;Deng M
对于泡桐对干旱的响应和适应仍然知之甚少。为了研究这一问题，利用Illumina Genome Analyzer IIx分析系统对4份芦苇种质（2份二倍体和2份同源四倍体）在水分胁迫下的转录组进行了分析。本研究的目的是鉴定可能参与芦苇对水分胁迫响应的代谢途径基因。盆栽幼苗进行充分浇水的条件和干旱胁迫，分别。超过2.9亿个原始转录物读数被组装成111，660个单基因，平均长度为1013 bp。对unigenes进行了直向群聚类、基因本体和京都基因和基因组百科全书注释分析。许多差异表达的基因和几个代谢途径进行了鉴定。实时定量聚合酶链反应用于验证14个基因的表达模式。我们的研究确定了干旱胁迫诱导的芦苇基因表达的改变，并提供了该物种干旱响应基因和途径的全面图谱。据我们所知，这是第一个公开的全球转录组研究P. australis。本研究为该物种提供了宝贵的遗传资源。
The response and adaptation to drought remains poorly understood for Paulownia australis. To investigate this issue, transcriptome profiling of four P. australis accessions (two diploid and the other two autotetraploid) under water stress condition were studied using Illumina Genome Analyzer IIx analysis. The current study aimed to identify genes of P. australis metabolism pathways that might be involved in this plant’s response to water deficit. Potted seedlings were subjected to well-watered conditions and drought stress, respectively. More than 290 million raw transcript reads were assembled into 111,660 unigenes, with a mean length of 1013 bp. Clusters of orthologous groups, gene ontology and the Kyoto Encyclopedia of Genes and Genomes annotations analyses were performed on the unigenes. Many differentially expressed genes and several metabolic pathways were identified. Quantitative real-time polymerase chain reaction was used to verify the expression patterns of 14 genes. Our study identified altered gene expression in P. australis induced by drought stress and provided a comprehensive map of drought-responsive genes and pathways in this species. To our knowledge, this is the first publicly available global transcriptome study of P. australis. This study provides a valuable genetic resource for this species.
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影响因子：
3
作者：
Broberg, P
通讯作者：
Broberg, P
影响因子：
5.1
作者：
Dubos, C;Plomion, C
通讯作者：
Plomion, C
影响因子：
7.3
作者：
Assmann, SM;Snyder, JA;Lee, YRJ
通讯作者：
Lee, YRJ
影响因子：
11.4
作者：
Kushiro, T;Okamoto, M;Nambara, E
通讯作者：
Nambara, E
影响因子：
7.4
作者：
Okamoto, M;Kuwahara, A;Nambara, E
通讯作者：
Nambara, E