Anisotropic engineered heart tissue made from laser-cut decellularized myocardium.
Anisotropic engineered heart tissue made from laser-cut decellularized myocardium.
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DOI：
10.1038/srep32068
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发表时间：
2016-08-30
影响因子：
4.6
通讯作者：
Campbell SG
中科院分区：
文献类型：
--
作者：
Schwan J;Kwaczala AT;Ryan TJ;Bartulos O;Ren Y;Sewanan LR;Morris AH;Jacoby DL;Qyang Y;Campbell SG
We have developed an engineered heart tissue (EHT) system that uses laser-cut sheets of decellularized myocardium as scaffolds. This material enables formation of thin muscle strips whose biomechanical characteristics are easily measured and manipulated. To create EHTs, sections of porcine myocardium were laser-cut into ribbon-like shapes, decellularized, and mounted in specialized clips for seeding and culture. Scaffolds were first tested by seeding with neonatal rat ventricular myocytes. EHTs beat synchronously by day five and exhibited robust length-dependent activation by day 21. Fiber orientation within the scaffold affected peak twitch stress, demonstrating its ability to guide cells toward physiologic contractile anisotropy. Scaffold anisotropy also made it possible to probe cellular responses to stretch as a function of fiber angle. Stretch that was aligned with the fiber direction increased expression of brain natriuretic peptide, but off-axis stretches (causing fiber shear) did not. The method also produced robust EHTs from cardiomyocytes derived from human embryonic stem cells and induced pluripotent stem cells (hiPSC). hiPSC-EHTs achieved maximum peak stress of 6.5 mN/mm2 and twitch kinetics approaching reported values from adult human trabeculae. We conclude that laser-cut EHTs are a viable platform for novel mechanotransduction experiments and characterizing the biomechanical function of patient-derived cardiomyoctyes.
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影响因子：
5
作者：
Ford SJ;Mamidi R;Jimenez J;Tardiff JC;Chandra M
通讯作者：
Chandra M
影响因子：
9.5
作者：
Hirt MN;Sörensen NA;Bartholdt LM;Boeddinghaus J;Schaaf S;Eder A;Vollert I;Stöhr A;Schulze T;Witten A;Stoll M;Hansen A;Eschenhagen T
通讯作者：
Eschenhagen T
影响因子：
14
作者：
Feinberg, Adam W.;Alford, Patrick W.;Jin, Hongwei;Ripplinger, Crystal M.;Werdich, Andreas A.;Sheehy, Sean P.;Grosberg, Anna;Parker, Kevin Kit
通讯作者：
Parker, Kevin Kit
影响因子：
6.1
作者：
Grosberg A;Alford PW;McCain ML;Parker KK
通讯作者：
Parker KK
影响因子：
4.1
作者：
Boudou, Thomas;Legant, Wesley R.;Chen, Christopher S.
通讯作者：
Chen, Christopher S.