Measuring Expression Levels of Endogenous Gli Genes by Immunoblotting and Real-Time PCR.
Measuring Expression Levels of Endogenous Gli Genes by Immunoblotting and Real-Time PCR.
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通过免疫印迹和实时 PCR 测量内源 Gli 基因的表达水平。
DOI：
10.1007/978-1-4939-2772-2_8
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Rohatgi,Rajat
中科院分区：
文献类型：
--
作者：
Niewiadomski,Pawel;Rohatgi,Rajat
Gli proteins are transcription factors that mediate the transcriptional effects of Hedgehog signaling in vertebrates. The activities of Gli2 and Gli3 are regulated primarily by posttranslational modifications, while Gli1 is mostly regulated at the transcriptional level. Detection of endogenous Gli proteins had been hampered by lack of good antibodies, but this problem has been mostly resolved in recent years. In this chapter we describe methods of detecting expression of endogenousGligenes in whole-cell lysates and in subcellular fractions and also provide protocols for the measurement of Gli mRNA levels by quantitative real-time reverse transcriptase PCR (qPCR).
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DOI：
--
发表时间：
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期刊：
Neurobehavioral toxicology and teratology
影响因子：
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作者：
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通讯作者：
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作者：
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DOI：
--
发表时间：
1986
期刊：
Neurobehavioral toxicology and teratology
影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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