Insights and prospects for ion mobility-mass spectrometry in clinical chemistry.
Insights and prospects for ion mobility-mass spectrometry in clinical chemistry.
复制标题
离子迁移率-质谱仪在临床化学中的应用展望。
DOI：
10.1080/14789450.2022.2026218
复制
发表时间：
2022-01
影响因子：
3.4
通讯作者：
McLean JA
中科院分区：
文献类型：
--
作者：
Koomen DC;May JC;McLean JA
离子迁移率质谱是临床环境中的一种新兴技术，用于复杂生物样品的高通量和高置信度分子表征。离子迁移谱法可以提供基于分子结构的异构体分离，其能力通过提供增强的分辨能力的技术发展而增加。集成多个分离维度，如液相色谱-离子迁移率-质谱（LC-IM-MS），可显著增强高精度临床测量的分子干扰缓解能力。LC-IM-MS多维分离在分子分析中提供更好的选择性和灵敏度。通过补充离子迁移率分析改进了组织的质谱成像以告知空间分子分布。手术环境中的生物标志物鉴定通过使用质谱的术中生化分析来增强，并且有望与离子迁移谱集成。高分辨率离子迁移率的新前景是提高分析能力，如区分异构体化合物。离子迁移率-质谱法在异构体鉴定、分子成像和临床环境中的术中肿瘤边缘描绘领域具有许多前景。提供这些优点的同时保持快速分析时间和随后的高通量。高分辨率离子迁移率将进一步增强这些优势，特别是对于需要高置信度同量异位素选择性和检测的分析。
Ion mobility-mass spectrometry is an emerging technology in the clinical setting for high throughput and high confidence molecular characterization from complex biological samples. Ion mobility spectrometry can provide isomer separations on the basis of molecular structure, the ability of which is increasing through technological developments that afford enhanced resolving power. Integrating multiple separation dimensions, such as liquid chromatography-ion mobility-mass spectrometry (LC-IM-MS) provide dramatic enhancements in the mitigation of molecular interferences for high accuracy clinical measurements. Multidimensional separations with LC-IM-MS provide better selectivity and sensitivity in molecular analysis. Mass spectrometry imaging of tissues to inform spatial molecular distribution is improved by complementary ion mobility analyses. Biomarker identification in surgical environments is enhanced by intraoperative biochemical analysis with mass spectrometry and holds promise for integration with ion mobility spectrometry. New prospects in high resolving power ion mobility are enhancing analysis capabilities, such as distinguishing isomeric compounds. Ion mobility-mass spectrometry holds many prospects for the field of isomer identification, molecular imaging, and intraoperative tumor margin delineation in clinical settings. These advantages are afforded while maintaining fast analysis times and subsequently high throughput. High resolving power ion mobility will enhance these advantages further, in particular for analyses requiring high confidence isobaric selectivity and detection.
登录
查看更多内容
DOI：
10.1007/s13361-019-02288-2
发表时间：
2019-11-01
影响因子：
3.2
作者：
Dodds, James N.;Baker, Erin S.
通讯作者：
Baker, Erin S.
影响因子：
1.3
作者：
COHEN, MJ;KARASEK, FW
通讯作者：
KARASEK, FW
影响因子：
17.1
作者：
Balog, Julia;Sasi-Szabo, Laszlo;Takats, Zoltan
通讯作者：
Takats, Zoltan
影响因子：
7.4
作者：
Davis, Don E., Jr.;Leaptrot, Katrina L.;Koomen, David C.;May, Jody C.;Cavalcanti, Gustavo de A.;Padilha, Monica C.;Pereira, Henrique M. G.;McLean, John A.
通讯作者：
McLean, John A.
影响因子：
2.2
作者：
Badoud, Flavia;Guillarme, Davy;Veuthey, Jean-Luc
通讯作者：
Veuthey, Jean-Luc