Computationally designed peptides for self-assembly of nanostructured lattices.
Computationally designed peptides for self-assembly of nanostructured lattices.
复制标题
计算设计的肽用于纳米结构晶格的自组装。
DOI：
10.1126/sciadv.1600307
复制
发表时间：
2016-09
期刊：
影响因子：
13.6
通讯作者：
Saven JG
中科院分区：
文献类型：
--
作者：
Zhang HV;Polzer F;Haider MJ;Tian Y;Villegas JA;Kiick KL;Pochan DJ;Saven JG
肽晶格是使用计算方法设计的，并通过调节自组装溶液条件来控制。折叠肽呈现由其氨基酸序列指定的复杂外表面，并且这些表面的控制提供了自组装材料的高精度路线。肽结构的复杂性和非共价相互作用的微妙性使得预定纳米结构的设计变得困难。计算方法可以促进这种设计，并在这里使用，以确定29个残基的肽，形成四螺旋束，反过来，作为构建模块的晶格形成材料。设计了四个不同的组件。肽束外部氨基酸被设计在三个不同的interbundle晶格的上下文中，除了一个设计，以产生在溶液中分离的束。溶液组装产生了三种不同类型的晶格形成材料，这些材料与每个序列设计中使用的所选晶格表现出不同程度的一致性。透射电子显微镜显示了片状纳米材料的纳米结构。相比之下，设计用于形成分离的可溶性四聚体束的肽序列保持分散，并且不形成任何更高阶组装的纳米结构。小角中子散射证实了与设计尺寸的可溶性束的形成。在晶格形成纳米结构中，溶液组装过程对于溶液条件（pH和温度）的变化和计算设计的肽的共价修饰是稳健的。溶液条件可用于控制组装体的微米级形态。研究结果表明，通过仔细控制分子结构和溶液条件，单个肽基序可以是通用的，足以在许多长度尺度（1至1000 nm）上产生广泛的自组装晶格形态。
Peptide lattices were designed using computational methods and controlled by tuning self-assembly solution conditions. Folded peptides present complex exterior surfaces specified by their amino acid sequences, and the control of these surfaces offers high-precision routes to self-assembling materials. The complexity of peptide structure and the subtlety of noncovalent interactions make the design of predetermined nanostructures difficult. Computational methods can facilitate this design and are used here to determine 29-residue peptides that form tetrahelical bundles that, in turn, serve as building blocks for lattice-forming materials. Four distinct assemblies were engineered. Peptide bundle exterior amino acids were designed in the context of three different interbundle lattices in addition to one design to produce bundles isolated in solution. Solution assembly produced three different types of lattice-forming materials that exhibited varying degrees of agreement with the chosen lattices used in the design of each sequence. Transmission electron microscopy revealed the nanostructure of the sheetlike nanomaterials. In contrast, the peptide sequence designed to form isolated, soluble, tetrameric bundles remained dispersed and did not form any higher-order assembled nanostructure. Small-angle neutron scattering confirmed the formation of soluble bundles with the designed size. In the lattice-forming nanostructures, the solution assembly process is robust with respect to variation of solution conditions (pH and temperature) and covalent modification of the computationally designed peptides. Solution conditions can be used to control micrometer-scale morphology of the assemblies. The findings illustrate that, with careful control of molecular structure and solution conditions, a single peptide motif can be versatile enough to yield a wide range of self-assembled lattice morphologies across many length scales (1 to 1000 nm).
登录
查看更多内容
影响因子：
5.6
作者：
Grigoryan G;Degrado WF
通讯作者：
Degrado WF
DOI：
10.1016/j.str.2005.10.010
发表时间：
2006-03
期刊：
Structure (London, England : 1993)
影响因子：
--
作者：
Deng Y;Liu J;Zheng Q;Eliezer D;Kallenbach NR;Lu M
通讯作者：
Lu M
影响因子：
5.6
作者：
Calhoun, JR;Kono, H;Saven, JG
通讯作者：
Saven, JG
DOI：
10.1073/pnas.1112595109
发表时间：
2012-05-08
影响因子：
11.1
作者：
Lanci, Christopher J.;MacDermaid, Christopher M.;Saven, Jeffery G.
通讯作者：
Saven, Jeffery G.
影响因子：
6
作者：
Gammer, C.;Mangler, C.;Karnthaler, H. P.
通讯作者：
Karnthaler, H. P.