Membrane tension maintains cell polarity by confining signals to the leading edge during neutrophil migration.
Membrane tension maintains cell polarity by confining signals to the leading edge during neutrophil migration.
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DOI：
10.1016/j.cell.2011.10.050
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发表时间：
2012-01-20
期刊：
影响因子：
64.5
通讯作者：
Weiner OD
中科院分区：
文献类型：
--
作者：
Houk AR;Jilkine A;Mejean CO;Boltyanskiy R;Dufresne ER;Angenent SB;Altschuler SJ;Wu LF;Weiner OD
关于中性粒细胞和其他细胞在迁移过程中如何建立肌动蛋白组装的单一区域，人们知之甚少。一个普遍的假设是，前缘通过产生长程扩散抑制剂或隔离必要的极性成分来防止形成额外的前沿。我们使用形态学扰动，细胞切断实验，和计算机模拟表明，基于扩散的机制是不足够的长距离抑制的伪足。相反，质膜张力可以作为中性粒细胞的长期抑制剂。我们发现，膜张力增加一倍，在前缘突起，增加张力是足以长距离抑制肌动蛋白组装和Rac激活。此外，降低膜张力导致均匀的肌动蛋白组装。我们认为，张力，而不是可扩散的分子产生或隔离在前沿，是远程抑制的主要来源，限制了现有的战线的蔓延，并防止形成二级战线。
Little is known about how neutrophils and other cells establish a single zone of actin assembly during migration. A widespread assumption is that the leading edge prevents formation of additional fronts by generating long-range diffusible inhibitors or by sequestering essential polarity components. We use morphological perturbations, cell severing experiments, and computational simulations to show that diffusion-based mechanisms are not sufficient for long-range inhibition by the pseudopod. Instead, plasma membrane tension could serve as a long-range inhibitor in neutrophils. We find that membrane tension doubles during leading edge protrusion, and increasing tension is sufficient for long-range inhibition of actin assembly and Rac activation. Furthermore, reducing membrane tension causes uniform actin assembly. We suggest that tension, rather than diffusible molecules generated or sequestered at the leading edge, is the dominant source of long-range inhibition that constrains the spread of the existing front and prevents the formation of secondary fronts.
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影响因子：
64.8
作者：
Mayer, Mirjam;Depken, Martin;Grill, Stephan W.
通讯作者：
Grill, Stephan W.
影响因子：
64.8
作者：
Keren, Kinneret;Pincus, Zachary;Theriot, Julie A.
通讯作者：
Theriot, Julie A.
DOI：
10.1083/jcb.200201105
发表时间：
2002-07-08
期刊：
The Journal of cell biology
影响因子：
--
作者：
Katsumi A;Milanini J;Kiosses WB;del Pozo MA;Kaunas R;Chien S;Hahn KM;Schwartz MA
通讯作者：
Schwartz MA
DOI：
10.1083/jcb.95.3.960
发表时间：
1982-12
期刊：
The Journal of cell biology
影响因子：
--
作者：
Malawista SE;De Boisfleury Chevance A
通讯作者：
De Boisfleury Chevance A
影响因子：
4.3
作者：
Jilkine A;Edelstein-Keshet L
通讯作者：
Edelstein-Keshet L