The cytokineplast: purified, stable, and functional motile machinery from human blood polymorphonuclear leukocytes.
The cytokineplast: purified, stable, and functional motile machinery from human blood polymorphonuclear leukocytes.
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DOI：
10.1083/jcb.95.3.960
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发表时间：
1982-12
期刊：
影响因子：
--
通讯作者：
De Boisfleury Chevance A
中科院分区：
文献类型：
--
作者：
Malawista SE;De Boisfleury Chevance A
我们检测了人血多形核白细胞(PMN，粒细胞)在短暂加热下形成的可移动的、化学活性的、无核的碎片。这些颗粒稀少的碎片是以前的原足类(前锋，片状脂肪)，它们与细胞主体解偶联，离开细胞，最初伴随着连接的细丝断裂并自我密封。这种细丝通常的随机取向可以通过细胞在趋化肽N-甲酰甲硫基亮氨酸苯丙氨酸(F-Met-Leu-Phe)(2x10(-9)M-1x10(-8))的梯度中的预取向来控制。细胞松弛素B，2.5-5μg/ml，阻止碎片形成；秋水仙碱，10(-5)M，不能。在扫描电子显微镜下，碎片起皱，细胞体变圆，相当光滑。在透射电子显微镜下，碎片含有微丝，但没有中心粒和微管。像完整的细胞一样，结合的和游离的碎片都可以对被激光微照射(趋死性)破坏的红细胞做出化学反应；自由的无核碎片可能会反复这样做，即使在环境温度下保存了一夜之后也是如此。我们建议将其命名为细胞分裂质，这是运动器官这种自净的结果。运动机械离开粒细胞需要将大部分细胞锚定在玻璃上并解偶联，这可能涉及到热诱导的中心体功能障碍。在中心体区加热后的超微结构研究中，中心粒结构保持不变，但中心粒周围嗜欧物质浓缩，微管稀疏。这些变化在所检查的三种血细胞中均可发现：中性粒细胞、嗜酸性粒细胞和单核细胞。在这些细胞中，前两个在我们的条件下会产生碎片；速度较慢的单核细胞则不会。解偶联进一步与中心体功能障碍有关，因为观察到秋水仙素处理的粒细胞(10(-5)M，以破坏中心体的传出臂)在较少的热量后产生碎片。如果动力和取向主要从细胞器指定--不包括前锋，那么PMN中的内质流是一种追赶现象，微管并不提供运动的载体，而是稳定和定向“包袱”(核、颗粒质)--即它们防止鱼尾。此外，中心体产生的限制现在可以扩展到包括作为细胞组成部分的移动机械的维护。
We examined the formation of motile, chemotactically active, anucleate fragments from human blood polymorphonuclear leukocytes (PMN, granulocytes), induced by the brief application of heat. These granule-poor fragments are former protopods (leading fronts, lamellipodia) that become uncoupled from the main body of the cell and leave it, at first with a connecting filament that breaks and seals itself. The usual random orientation of such filaments can be controlled by preorientation of cells in a gradient of the chemotactic peptide, N-formylmethionylleucylphenylalanine (F-Met-Leu-Phe) (2x10(-9) M- 1x10(-8)). Cytochalsin B, 2.5-5 μg/ml, prevents fragment formation; colchicine, 10(-5) M, does not. In scanning electron micrographs, fragments are ruffled and the cell body rounded up and rather smooth. In transmission electron micrographs, fragments contain microfilaments but lack centrioles and microtubules. Like intact cells, both bound and free fragments can respond chemotactically to an erythrocyte destroyed by laser microirradiation (necrotaxis); the free, anucleate fragments may do so repeatedly, even after having been held overnight at ambient temperatures. We propse the name cytokineplast for the result of this self-purification of motile apparatus. The exodus of the motile machinery from the granulocyte requires anchoring of the bulk of the cell to glass and uncoupling, which may involve heat-induced dysfunction of the centrosome. In ultrastructural studies of the centrosomal region after heat, centriolar structure remains intact, but pericentriolar osmiophilic material appears condensed, and microtubules are sparse. These changes are found in all three blood cell types examined: PMN, eosinophil, and monocyte. Of these, the first two make fragments under our conditions; the more sluggish monocyte does not. Uncoupling is further linked to centrosomal dysfunction by the observation that colchicines-treated granulocytes (10(-5)M, to destroy the centrosome’s efferent arm) make fragments after less heat than controls. If motive force and orientation are specified mainly from the organelle-excluding leading front, then endoplasmic streaming in PMN is a catch-up phenomenon, and microtubules do not provide the vector of locomotion but rather stabilize and orient the “baggage” (nucleus, granuloplasm)—i.e., they prevent fishtailing. Moreover, constraints emanating from the centrosome may now be extended to include, maintenance of the motile machinery as an integral part of the cell.
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影响因子：
--
作者：
MALAWISTA, SE
通讯作者：
MALAWISTA, SE
影响因子：
15.9
作者：
MALAWISTA, SE;BODEL, PT
通讯作者：
BODEL, PT
影响因子：
3.7
作者：
BANDMANN, U;RYDGREN, L;NORBERG, B
通讯作者：
NORBERG, B
DOI：
10.1083/jcb.75.3.941
发表时间：
1977-12
期刊：
The Journal of cell biology
影响因子：
--
作者：
Davies WA;Stossel TP
通讯作者：
Stossel TP
影响因子：
56.9
作者：
MALAWIST.SE;BENSCH, KG
通讯作者：
BENSCH, KG