Identification and fine mapping of qGR6.2, a novel locus controlling rice seed germination under salt stress.
Identification and fine mapping of qGR6.2, a novel locus controlling rice seed germination under salt stress.
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盐胁迫下控制水稻种子萌发的新位点qGR6.2的鉴定和精细定位
DOI：
10.1186/s12870-020-02820-7
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发表时间：
2021-01-09
影响因子：
5.3
通讯作者：
Cheng J
中科院分区：
文献类型：
--
作者：
Zeng P;Zhu P;Qian L;Qian X;Mi Y;Lin Z;Dong S;Aronsson H;Zhang H;Cheng J
水稻生长经常受到盐分的影响。高盐胁迫对水稻种子萌发和成苗有明显的抑制作用。随着水稻直播技术在亚洲的推广，提高水稻种子在盐胁迫下的发芽率成为水稻育种的关键。以WJZ/Nip（japonica，Nipponbare）//Nip杂交后代的BC 1F 2群体为材料，对水稻种子在H2O和300 mM NaCl条件下的发芽率（GR）和发芽指数（GI）进行QTL定位。共检测到13个QTL，其中10个在水处理条件下，9个在盐处理条件下。在两种条件下同时检测到qGR 6. 1、qGR 8. 1、qGR 8. 2、qGR 10. 1、qGR 10. 2和qGI 10. 1 6个QTL。在盐胁迫下，在种子萌发的不同时间点检测到3个控制GR的QTL，分别为qGR 6. 2、qGR 10. 1和qGR 10. 2，它们分别与控制GI的qGI 6. 2、qGI 10. 1和qGI 10. 2具有相同的染色体区域。qGR6.2为盐胁迫下的主效QTL，对表型变异的贡献率超过20%。通过BC 2F 2群体验证了qGR6.2的有效性，并将其缩小到65.9-kb的区域，预测了11个候选基因。利用基因芯片数据库，筛选出5个在种子萌发期转录丰度较高的候选基因，其中LOC_Os06g10650和LOC_Os06g10710在种子吸胀后差异表达。RT-qPCR结果表明，LOC_Os06g10650基因在盐胁迫下的萌发过程中，在两个亲本中的表达量均显著上调，其中WJZ的表达量高于Nip。这些结果表明，编码酪氨酸磷酸酶家族蛋白的LOC_Os06g10650可能是qGR 6. 2的致病候选基因。一个主要的耐盐特异性QTLqGR6.2被精细定位在65.9kb的区域。这些结果为利用分子标记辅助选择（MAS）提高盐胁迫下水稻种子发芽率提供了遗传基础。
BackgroundRice growth is frequently affected by salinity. When exposed to high salinity, rice seed germination and seedling establishment are significantly inhibited. With the promotion of direct-seeding in Asia, improving rice seed germination under salt stress is crucial for breeding.ResultsIn this study, anindicalandrace Wujiaozhan (WJZ) was identified with high germinability under salt stress. A BC1F2population derived from the crossing WJZ/Nip (japonica, Nipponbare)//Nip, was used to quantitative trait loci (QTL) mapping for the seed germination rate (GR) and germination index (GI) under H2O and 300 mM NaCl conditions. A total of 13 QTLs were identified, i.e. ten QTLs under H2O conditions and nine QTLs under salt conditions. Six QTLs,qGR6.1,qGR8.1,qGR8.2,qGR10.1,qGR10.2andqGI10.1were simultaneously identified under two conditions. Under salt conditions, three QTLs,qGR6.2,qGR10.1andqGR10.2for GR were identified at different time points during seed germination, which shared the same chromosomal region withqGI6.2,qGI10.1andqGI10.2for GI respectively. TheqGR6.2accounted for more than 20% of phenotypic variation under salt stress, as the major effective QTL. Furthermore,qGR6.2was verified via the BC2F2population and narrowed to a 65.9-kb region with eleven candidate genes predicted. Based on the microarray database, five candidate genes were found with high transcript abundances at the seed germination stage, of whichLOC_Os06g10650andLOC_Os06g10710were differentially expressed after seed imbibition. RT-qPCR results showed the expression ofLOC_Os06g10650was significantly up-regulated in two parents with higher levels in WJZ than Nip during seed germination under salt conditions. Taken together, it suggests thatLOC_Os06g10650, encoding tyrosine phosphatase family protein, might be the causal candidate gene forqGR6.2.ConclusionsIn this study, we identified 13 QTLs from a landrace WJZ that confer seed germination traits under H2O and salt conditions. A major salt-tolerance-specific QTLqGR6.2was fine mapped to a 65.9-kb region. Our results provide information on the genetic basis of improving rice seed germination under salt stress by marker-assisted selection (MAS).
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影响因子：
5.4
作者：
Chen, X;Temnykh, S;McCouch, SR
通讯作者：
McCouch, SR
DOI：
10.1073/pnas.0805303105
发表时间：
2008-08-26
影响因子：
11.1
作者：
Fujino, Kenji;Sekiguchi, Hiroshi;Yano, Masahiro
通讯作者：
Yano, Masahiro
影响因子：
4.8
作者：
Chai Lu;Zhang Jian;Li Zhi-kang
通讯作者：
Li Zhi-kang
DOI：
10.4236/ajps.2012.37116
发表时间：
2012-01-01
期刊：
American Journal of Plant Sciences
影响因子：
--
作者：
Huyen, L. T. N.;Cuc, L. M.;Ham, L. H.
通讯作者：
Ham, L. H.
DOI：
10.1016/b978-0-12-802389-1.00007-1
发表时间：
2016-01-01
期刊：
CHROMATIN SIGNALING AND DISEASES
影响因子：
--
作者：
Morrison, E. A.;Musselman, C. A.
通讯作者：
Musselman, C. A.