Efficient Detection of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) from Exhaled Breath.
Efficient Detection of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) from Exhaled Breath.
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DOI：
10.1016/j.jmoldx.2021.09.005
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发表时间：
2021-12
期刊：
影响因子：
--
通讯作者：
Fairbrother WG
中科院分区：
文献类型：
--
作者：
Duan C;Buerer L;Wang J;Kaplan S;Sabalewski G;Jay GD;Monaghan SF;Arena AE;Fairbrother WG
严重急性呼吸道综合征-冠状病毒2（SARS-CoV-2）通过呼出气中的空气传播颗粒传播，在部分患者中引起严重呼吸道疾病，即2019冠状病毒病（COVID-19）。用于SARS-CoV-2检测的样本通常通过鼻咽拭子采集，通过PCR检测病毒;然而，患者可以在感染后3个月内检测为阳性。如果没有检测呼吸中SARS-CoV-2的能力，就不可能了解感染者传播的风险。为了检测呼吸中的病毒，开发了Bubbler-一种呼吸测醉器，它将SARS-CoV-2颗粒的RNA逆转录成样本特异性条形码cDNA。在一项对70名住院患者的研究中，Bubbler比替代品更能预测下呼吸道受累（胸部X线检查异常），而且侵入性更小。使用Bubbler测试的样本的SARS-CoV-2 RNA含量是舌拭子样本的三倍，这意味着病毒颗粒是直接采样的。条形码使能的Bubbler用于在大批量合并样品中同时诊断，每个样品的检测下限为334个基因组拷贝。通过测序进行诊断可以提供额外的信息，如病毒载量和毒株身份。Bubbler被配置为对空气中循环的水滴中的核酸进行采样，证明了其在环境监测中的潜力以及充分通风的保护作用。
Severe acute respiratory syndrome–coronavirus 2 (SARS-CoV-2) is transmitted through airborne particles in exhaled breath, causing severe respiratory disease, coronavirus disease–2019 (COVID-19), in some patients. Samples for SARS-CoV-2 testing are typically collected by nasopharyngeal swab, with the virus detected by PCR; however, patients can test positive for 3 months after infection. Without the capacity to assay SARS-CoV-2 in breath, it is not possible to understand the risk for transmission from infected individuals. To detect virus in breath, the Bubbler—a breathalyzer that reverse-transcribes RNA from SARS-CoV-2 particles into a sample-specific barcoded cDNA—was developed. In a study of 70 hospitalized patients, the Bubbler was both more predictive of lower respiratory tract involvement (abnormal chest X-ray) and less invasive than alternatives. Samples tested using the Bubbler were threefold more enriched for SARS-CoV-2 RNA than were samples from tongue swabs, implying that virus particles were being directly sampled. The barcode-enabled Bubbler was used for simultaneous diagnosis in large batches of pooled samples at a lower limit of detection of 334 genomic copies per sample. Diagnosis by sequencing can provide additional information, such as viral load and strain identity. The Bubbler was configured to sample nucleic acids in water droplets circulating in air, demonstrating its potential in environmental monitoring and the protective effect of adequate ventilation.
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作者：
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DOI：
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影响因子：
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作者：
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