Fast multicolor 3D imaging using aberration-corrected multifocus microscopy.

Fast multicolor 3D imaging using aberration-corrected multifocus microscopy.
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DOI:
10.1038/nmeth.2277
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发表时间:
2013-01
期刊:
影响因子:
48
通讯作者:
Gustafsson, Mats G. L.
Gustafsson, Mats G. L.
中科院分区:
生物学1区
文献类型:
--
作者:
Abrahamsson, Sara;Chen, Jiji;Hajj, Bassam;Stallinga, Sjoerd;Katsov, Alexander Y.;Wisniewski, Jan;Mizuguchi, Gaku;Soule, Pierre;Mueller, Florian;Darzacq, Claire Dugast;Darzacq, Xavier;Wu, Carl;Bargmann, Cornelia I.;Agard, David A.;Dahan, Maxime;Gustafsson, Mats G. L.

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三维(3D)显微镜数据的常规采集需要顺序z扫描,并且通常太慢而无法捕获生物事件。我们报告了一种新的像差校正多焦点显微镜方法,能够产生9个2D图像的即时焦点堆栈。该多焦点系统配备了落射荧光显微镜,能够以单分子灵敏度实现多种颜色的高分辨率3D成像,速度受单个图像的相机读出时间的限制。
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