Fast multicolor 3D imaging using aberration-corrected multifocus microscopy.
Fast multicolor 3D imaging using aberration-corrected multifocus microscopy.
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DOI:
10.1038/nmeth.2277
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发表时间:
2013-01
期刊:
影响因子:
48
通讯作者:
Gustafsson, Mats G. L.
中科院分区:
文献类型:
--
作者:
Abrahamsson, Sara;Chen, Jiji;Hajj, Bassam;Stallinga, Sjoerd;Katsov, Alexander Y.;Wisniewski, Jan;Mizuguchi, Gaku;Soule, Pierre;Mueller, Florian;Darzacq, Claire Dugast;Darzacq, Xavier;Wu, Carl;Bargmann, Cornelia I.;Agard, David A.;Dahan, Maxime;Gustafsson, Mats G. L.
Conventional acquisition of three-dimensional (3D) microscopy data requires sequential z-scanning and is often too slow to capture biological events. We report a new aberration-corrected multi-focus microscopy method capable of producing an instant focal stack of nine 2D images. Appended to an epifluorescence microscope, the multi-focus system enables high-resolution 3D imaging in multiple colors with single molecule sensitivity, at speeds limited by the camera readout time of a single image.
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DOI:
10.1364/josaa.12.002145
发表时间:
1995-10-01
影响因子:
1.9
作者:
MAIT, JN
通讯作者:
MAIT, JN
DOI:
10.1073/pnas.0900245106
发表时间:
2009-03-03
影响因子:
11.1
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通讯作者:
Moerner, W. E.
影响因子:
3.9
作者:
Prabhat, P;Ram, S;Ober, RJ
通讯作者:
Ober, RJ
影响因子:
7.8
作者:
Pearson, C G;Maddox, P S;Salmon, E D;Bloom, K
通讯作者:
Bloom, K
影响因子:
3.4
作者:
Ram, Sripad;Prabhat, Prashant;Ober, Raimund J.
通讯作者:
Ober, Raimund J.