Presynaptic and postsynaptic origin of multicomponent extracellular waveforms at the endbulb of Held–spherical bushy cell synapse
Presynaptic and postsynaptic origin of multicomponent extracellular waveforms at the endbulb of Held–spherical bushy cell synapse
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Held 球状浓密细胞突触末端多分量细胞外波形的突触前和突触后起源
DOI：
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发表时间：
2010
影响因子：
3.4
通讯作者：
R. Rübsamen
中科院分区：
文献类型：
--
作者：
Marei Typlt;Martin D. Haustein;Beatrice Dietz;J. R. Steinert;Mirko Witte;B. Englitz;I. Milenkovic;C. Kopp‐Scheinpflug;I. Forsythe;R. Rübsamen
保持球形丛状细胞(SBC)突触的细胞外信号表现为三个波(‘P’、‘A’和‘B’)。缺少第三个成分(B)的信号经常被观察到，但由于每个成分的来源尚不确定，对这种缺乏B的解释一直存在争议：是没有释放递质，还是没有产生或传播动作电位？我们的目标是确定每一种成分的来源。我们在蒙古沙土鼠和Wistar大鼠身上结合了单单位和多单位的体外方法，并使用药理学工具来调节谷氨酸受体或电压门控钠通道。单个SBCs的细胞外和细胞内同步记录显示P成分起源于突触前，这与多电极阵列记录的局部场电位的数据一致。后两个成分(A和B)分别对应于SBC的兴奋性突触后电位(EPSP)和动作电位。这些结果可以清楚地解释体内的细胞外信号。我们的结论是，发生在终球-SBC突触交界处的动作电位故障主要反映了EPSP触发动作电位的故障，而不是突触传递的故障。这些数据为未来研究兴奋性和抑制性输入在使用生理刺激调制全功能神经系统的传递中的会聚提供了基础。
Extracellular signals from the endbulb of Held–spherical bushy cell (SBC) synapse exhibit up to three component waves (‘P’, ‘A’ and ‘B’). Signals lacking the third component (B) are frequently observed but as the origin of each of the components is uncertain, interpretation of this lack of B has been controversial: is it a failure to release transmitter or a failure to generate or propagate an action potential? Our aim was to determine the origin of each component. We combined single‐ and multiunit in vitro methods in Mongolian gerbils and Wistar rats and used pharmacological tools to modulate glutamate receptors or voltage‐gated sodium channels. Simultaneous extra‐ and intracellular recordings from single SBCs demonstrated a presynaptic origin of the P‐component, consistent with data obtained with multielectrode array recordings of local field potentials. The later components (A and B) correspond to the excitatory postsynaptic potential (EPSP) and action potential of the SBC, respectively. These results allow a clear interpretation of in vivo extracellular signals. We conclude that action potential failures occurring at the endbulb–SBC synaptic junction largely reflect failures of the EPSP to trigger an action potential and not failures of synaptic transmission. The data provide the basis for future investigation of convergence of excitatory and inhibitory inputs in modulating transmission at a fully functional neuronal system using physiological stimulation.
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收敛对尖峰时序影响的动态钳位分析。
DOI：
10.1152/jn.01308.2004
发表时间：
2005
期刊：
Journal of neurophysiology.
影响因子：
--
作者：
Xu-Friedman,MatthewA;Regehr,WadeG
通讯作者：
Regehr,WadeG
影响因子：
3.7
作者：
Englitz B;Tolnai S;Typlt M;Jost J;Rübsamen R
通讯作者：
Rübsamen R
影响因子：
2.5
作者：
Gold, C;Henze, DA;Buzsáki, G
通讯作者：
Buzsáki, G
影响因子：
2.5
作者：
Henze, DA;Borhegyi, Z;Buzsáki, G
通讯作者：
Buzsáki, G