16S rDNA analysis of periodontal plaque in chronic obstructive pulmonary disease and periodontitis patients.
16S rDNA analysis of periodontal plaque in chronic obstructive pulmonary disease and periodontitis patients.
复制标题
慢性阻塞性肺疾病和牙周炎患者牙周菌斑的16S rDNA分析
DOI：
10.1080/20002297.2017.1324725
复制
发表时间：
2017
影响因子：
4.5
通讯作者：
Yu L
中科院分区：
文献类型：
--
作者：
Wu X;Chen J;Xu M;Zhu D;Wang X;Chen Y;Wu J;Cui C;Zhang W;Yu L
摘要本研究调查了慢性阻塞性肺疾病（COPD）是否通过牙周微生物群与牙周炎相关，以及某些细菌是否影响牙周炎和COPD。此外，该研究还调查了患有COPD是否与牙周微生物群的丰富性和多样性减少有关。从105例患者中获得龈下菌斑。从55名COPD和50名非COPD参与者（有或无牙周炎）中分离细菌DNA。16S rRNA基因宏基因组测序用于表征微生物群并确定分类学分类。在非牙周炎患者中，患有COPD导致牙周微环境中细菌丰度和多样性下降。在COPD和牙周炎患者中，Dysgonomonas，Desulfobulbus和Catonella属以及四种物种（Porphyromonas endodontalis，Dysgonomonas wimpennyi，Catonella morbi和Prevotella intermedia）的增加表明这些牙周炎相关微生物群的增加可能与COPD有关。三个属（Johnsonella，Campylobacter和Oribacterium）与COPD相关，但与牙周炎无关。COPD患者中隐杆菌属、奥里杆菌属和链霉菌属的减少意味着这些属可能是与健康相关的属，并且这些属的减少可能与疾病有关。这些数据支持COPD通过这些显著变化的特定细菌与牙周炎相关的假设。
ABSTRACT This study investigated if chronic obstructive pulmonary disease (COPD) is correlated with periodontitis via periodontal microbiota and if certain bacteria affect periodontitis as well as COPD. Moreover, the study investigated whether suffering from COPD is associated with a decrease in the richness and diversity of periodontal microbiota. Subgingival plaque was obtained from 105 patients. Bacterial DNA was isolated from 55 COPD and 50 non-COPD participants (either with or without periodontitis). 16S rRNA gene metagenomic sequencing was used to characterize the microbiota and to determine taxonomic classification. In the non-periodontitis patients, suffering from COPD resulted in a decrease in bacteria richness and diversity in the periodontal microenvironment. An increase in the genera Dysgonomonas, Desulfobulbus, and Catonella and in four species (Porphyromonas endodontalis, Dysgonomonas wimpennyi, Catonella morbi, and Prevotella intermedia) in both COPD and periodontitis patients suggests that an increase in these periodontitis-associated microbiota may be related to COPD. Three genera (Johnsonella, Campylobacter, and Oribacterium) were associated with COPD but not with periodontitis. The decrease in the genera Arcanobacterium, Oribacterium, and Streptomyces in COPD patients implies that these genera may be health-associated genera, and the decrease in these genera may be related to disease. These data support the hypothesis that COPD is correlated with periodontitis via these significantly changed specific bacteria.
登录
查看更多内容
影响因子：
14.9
作者：
Klindworth A;Pruesse E;Schweer T;Peplies J;Quast C;Horn M;Glöckner FO
通讯作者：
Glöckner FO
影响因子：
168.9
作者：
Dickson, Robert P.;Martinez, Fernando J.;Huffnagle, Gary B.
通讯作者：
Huffnagle, Gary B.
DOI：
10.4103/0970-2113.85689
发表时间：
2011-10
期刊：
Lung India : official organ of Indian Chest Society
影响因子：
--
作者：
Godara N;Godara R;Khullar M
通讯作者：
Khullar M
与牙周炎和吸烟的影响相比，健康中的微生物群。
影响因子：
5.2
作者：
Camelo-Castillo AJ;Mira A;Pico A;Nibali L;Henderson B;Donos N;Tomás I
通讯作者：
Tomás I
影响因子：
3.7
作者：
Aagaard K;Riehle K;Ma J;Segata N;Mistretta TA;Coarfa C;Raza S;Rosenbaum S;Van den Veyver I;Milosavljevic A;Gevers D;Huttenhower C;Petrosino J;Versalovic J
通讯作者：
Versalovic J