Subgingival microbiota in health compared to periodontitis and the influence of smoking.
Subgingival microbiota in health compared to periodontitis and the influence of smoking.
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与牙周炎和吸烟的影响相比，健康中的微生物群。
DOI：
10.3389/fmicb.2015.00119
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发表时间：
2015
影响因子：
5.2
通讯作者：
Tomás I
中科院分区：
文献类型：
--
作者：
Camelo-Castillo AJ;Mira A;Pico A;Nibali L;Henderson B;Donos N;Tomás I
传统上，牙周炎的病因与三种细菌的联合有关——所谓的牙周病“红色复合体”——这是大多数诊断和治疗策略的目标。然而，其他物种也被发现与疾病严重程度相关。此外，吸烟对牙周微生物群的影响尚不清楚。在目前的论文中，使用16S焦磷酸测序技术研究了健康个体与慢性牙周炎患者牙龈下微生物群的组成，并研究了吸烟对牙周组成的影响。对82例患者的牙龈下细菌群落进行了采样：22例不吸烟的健康对照组，28例不吸烟的牙周患者和32例吸烟的牙周患者。牙周病患者的细菌多样性高于健康受试者，这可以解释为营养丰富的环境或免疫能力下降的结果。牙周患者显示出明显较高的患病率/相对丰度，但也有其他分类群，其作用尚未确定，应成为未来研究的目标。这些包括无氧舌虫、子弹虫、Desulfobulbus、Filifactor、Mogibacterium、Phocaeicola、Schwartzia或TM7。吸烟相关性牙周炎的微生物群落多样性较低，与非吸烟者不同，表明吸烟对牙周生态有影响。有趣的是，高测序覆盖率允许在所有健康个体中以低比例检测牙周病原体，这表明慢性牙周炎不能严格地被认为是一种传染病，而是多微生物生态失调的结果，其中微生物联合体比例的变化引发宿主的炎症和组织降解反应。
The etiology of periodontitis has traditionally been associated to a consortium of three bacterial species—the so-called “red-complex” of periodontal disease—which has been the target for most diagnostic and therapeutic strategies. However, other species have also been found to correlate with disease severity. In addition, the influence of smoking on periodontal microbiota is poorly understood. In the current manuscript, the composition of the subgingival microbiota in healthy individuals vs. patients with chronic periodontitis has been investigated using 16S pyrosequencing and the influence of smoking on periodontal composition has been examined. Subgingival bacterial communities were sampled from 82 patients: 22 non-smoking healthy controls, 28 non-smoking periodontal patients, and 32 smoking periodontal patients. Bacterial diversity was higher in periodontal patients than in healthy subjects, which could be interpreted as the consequence of a nutritionally richer environment or a reduced immune competence. Periodontal patients showed a significantly higher prevalence/relative abundance of “established” periopathogens but also other taxa whose role is not well-established and that should be targets for future research. These include Anaeroglobus, Bulleidia, Desulfobulbus, Filifactor, Mogibacterium, Phocaeicola, Schwartzia or TM7. The microbial community of smoking-associated periodontitis is less diverse and distinct from that of non-smokers, indicating that smoking has an influence on periodontal ecology. Interestingly, the high sequencing coverage allowed the detection at low proportions of periodontal pathogens in all healthy individuals, indicating that chronic periodontitis cannot be strictly considered an infectious disease but the outcome of a polymicrobial dysbiosis, where changes in the proportions of microbial consortia trigger the inflammatory and tissue-degradation responses of the host.
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影响因子：
3.7
作者：
Ge X;Rodriguez R;Trinh M;Gunsolley J;Xu P
通讯作者：
Xu P
影响因子：
14.9
作者：
Cole JR;Wang Q;Cardenas E;Fish J;Chai B;Farris RJ;Kulam-Syed-Mohideen AS;McGarrell DM;Marsh T;Garrity GM;Tiedje JM
通讯作者：
Tiedje JM
影响因子：
3.7
作者：
Hajishengallis G;Lamont RJ
通讯作者：
Lamont RJ
影响因子：
9.4
作者：
Kumar, PS;Griffen, AL;Leys, EJ
通讯作者：
Leys, EJ
影响因子：
6.7
作者：
Darby, I;Hodge, PJ;Kinane, DF
通讯作者：
Kinane, DF