Simultaneous Protective Immune Responses of Ducks against Duck Plague and Fowl Cholera by Recombinant Duck Enteritis Virus Vector Expressing Pasteurella multocida OmpH Gene.
Simultaneous Protective Immune Responses of Ducks against Duck Plague and Fowl Cholera by Recombinant Duck Enteritis Virus Vector Expressing Pasteurella multocida OmpH Gene.
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DOI：
10.3390/vaccines10081358
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发表时间：
2022-08-19
期刊：
影响因子：
7.8
通讯作者：
Sthitmatee, Nattawooti
中科院分区：
文献类型：
--
作者：
Apinda, Nisachon;Muenthaisong, Anucha;Chomjit, Paweena;Sangkakam, Kanokwan;Nambooppha, Boondarika;Rittipornlertrak, Amarin;Koonyosying, Pongpisid;Yao, Yongxiu;Nair, Venugopal;Sthitmatee, Nattawooti
鸭肠炎病毒和多杀性巴氏杆菌是鸭的主要病原体，分别在鸭和其他水禽种群中诱发鸭瘟和禽霍乱，导致高发病率和死亡率。使用含有多杀性巴氏杆菌外膜蛋白H（OmpH）的DEV减毒活疫苗进行免疫可以为鸭类提供针对这两种传染病的最有效保护。我们最近报道了使用 CRISPR/Cas9 基因编辑策略构建表达多杀性巴氏杆菌 ompH 基因的重组 DEV，目的是将其用作可同时预防两种感染的二价疫苗。在这里，我们描述了我们对两种重组 DEV-ompH 疫苗构建体诱导的全身免疫反应、效力和临床保护的调查结果，其中每个 ompH 基因各一个副本分别插入 DEV 基因组的 UL55-LORF11 和 UL44-44.5 基因间区域。我们的研究表明，ompH 基因的插入对 DEV 亲本病毒没有产生不利影响。此外，用 rDEV-ompH-UL55 和 rDEV-ompH-UL44 疫苗免疫的雏鸭诱导了多杀性巴氏杆菌 OmpH 特异性抗体和 DEV 特异性抗体的良好水平，并且完全免受这两种疾病的影响。体液和细胞免疫分析证实了两种重组疫苗的免疫原性，它们提供了针对 DEV 和多杀性巴氏杆菌的强烈免疫反应。这项研究不仅为了解鸭子对重组 DEV-ompH 疫苗的免疫反应提供了见解，而且还证明了使用表达细菌免疫原的病毒载体同时预防病毒和细菌感染的潜力。
Duck enteritis virus and Pasteurella multocida are major duck pathogens that induce duck plague and fowl cholera, respectively, in ducks and other waterfowl populations, leading to high levels of morbidity and mortality. Immunization with live attenuated DEV vaccine containing P. multocida outer membrane protein H (OmpH) can provide the most effective protection against these two infectious diseases in ducks. We have recently reported the construction of recombinant DEV expressing P. multocida ompH gene using the CRISPR/Cas9 gene editing strategy with the goal of using it as a bivalent vaccine that can simultaneously protect against both infections. Here we describe the findings of our investigation into the systemic immune responses, potency and clinical protection induced by the two recombinant DEV-ompH vaccine constructs, where one copy each of the ompH gene was inserted into the DEV genome at the UL55-LORF11 and UL44-44.5 intergenic regions, respectively. Our study demonstrated that the insertion of the ompH gene exerted no adverse effect on the DEV parental virus. Moreover, ducklings immunized with the rDEV-ompH-UL55 and rDEV-ompH-UL44 vaccines induced promising levels of P. multocida OmpH-specific as well as DEV-specific antibodies and were completely protected from both diseases. Analysis of the humoral and cellular immunity confirmed the immunogenicity of both recombinant vaccines, which provided strong immune responses against DEV and P. multocida. This study not only provides insights into understanding the immune responses of ducks to recombinant DEV-ompH vaccines but also demonstrates the potential for simultaneous prevention of viral and bacterial infections using viral vectors expressing bacterial immunogens.
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