Lactobacillus johnsonii BS15 combined with abdominal massage on intestinal permeability in rats with nonalcoholic fatty liver and cell biofilm repair.
Lactobacillus johnsonii BS15 combined with abdominal massage on intestinal permeability in rats with nonalcoholic fatty liver and cell biofilm repair.
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DOI：
10.1080/21655979.2021.1954134
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发表时间：
2021-12
期刊：
影响因子：
4.9
通讯作者：
Wang J
中科院分区：
文献类型：
--
作者：
Zhang W;Li H;Zhao N;Luo X;Liu S;Bao A;Chen Y;Wang H;Wang J;Wang J
本研究旨在分析从阿湖红原草原自制酸奶中分离出的约氏酸乳BS 15联合腹部按摩对非酒精性脂肪性肝病（NAFLD）大鼠肠道通透性及细胞生物膜修复的影响。将45只大鼠随机分为5组，其中4组采用高脂饲料喂养建立NAFLD模型。按治疗方法分为A组（乳酸菌组）、B组（腹部按摩组）、A + B组（两者联合组）、模型组（蒸馏水组）、正常组（蒸馏水组）。观察肝脏、小肠通透性等病理指标。模型组FITC-葡聚糖含量较正常组明显升高（P < 0.01），提示高脂饮食喂养的NAFLD大鼠肠道通透性增加。A组、B组、A + B组小肠通透性均较模型组明显降低（P < 0.01），其中A + B组作用最为明显。肝组织HE染色显示，联合用药可改善造模引起的肝细胞结构改变，恢复肠细胞的正常结构。乳酸菌联合腹部按摩治疗效果优于两种单独治疗，进一步促进NAFLD大鼠肠黏膜通透性，修复肝细胞生物被膜。结果初步验证了腹部按摩对肠黏膜通透性的干预作用，进一步揭示了腹部按摩通过改善肠黏膜屏障通透性治疗NAFLD的机制。
This study aimed to analyze the effect of lactobacillus johnsonii BS15 (isolation of homemade yogurt from Ahu Hongyuan Grassland) combined with abdominal massage on intestinal permeability in rats with nonalcoholic fatty liver disease (NAFLD) and cell biofilm repair. Forty-five rats were divided randomly into five groups, four of which were fed with high-fat diet to establish NAFLD models. According to the treatment methods, they were grouped into group A (lactic acid bacteria feeding), group B (abdominal massage), group A + B (a combination of the two methods), model group (distilled water feeding), and normal group (distilled water feeding). Then, the pathological indexes of liver and intestinal permeability were observed. FITC-Dextran content of the model group elevated markedly compared with normal group (P < 0.01), indicating that the intestinal permeability of NAFLD rats fed with high-fat diet increased. The intestinal permeability of groups A, B, and A + B was lower sharply than that of model group (P < 0.01), and the effect of group A + B was the most obvious. HE staining of liver tissues showed that combined treatment could improve structural changes in liver cells caused by modeling and restore the normal structure of intestinal cells. Lactobacillus combined with abdominal massage was better than two treatments alone, further promoting the permeability of intestinal mucosa in NAFLD rats and repair biofilm of hepatocytes. The results initially verified the intervention effect of abdominal massage on intestinal mucosal permeability, and further revealed the mechanism of abdominal massage in treatment of NAFLD by improving intestinal mucosal barrier permeability.
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影响因子：
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作者：
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