Protective Role of Probiotic Supplements in Hepatic Steatosis: A Rat Model Study.
Protective Role of Probiotic Supplements in Hepatic Steatosis: A Rat Model Study.
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DOI：
10.1155/2020/5487659
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发表时间：
2020
影响因子：
--
通讯作者：
Gholami A
中科院分区：
文献类型：
--
作者：
Azarang A;Farshad O;Ommati MM;Jamshidzadeh A;Heydari R;Abootalebi SN;Gholami A
治疗非酒精性脂肪肝（NAFLD）被认为是过去十年中公共卫生的优先事项之一。到目前为止，益生菌在控制NAFLD的体征和症状方面取得了可喜的成果。然而，寻找理想益生菌菌株的尝试仍在进行中。本研究旨在根据益生菌改善肝脂肪变性诱导大鼠组织病理学和氧化应激生物标志物的能力，在合适的益生菌菌株中找到最佳菌株。 最初，四种益生菌物种，包括乳杆菌（L.）嗜酸乳杆菌L.干酪湖（L. casei）罗伊氏杆菌和凝结芽孢杆菌，并制备成动物用冻干粉末。实验持续了50天。最初，通过在大鼠中过量摄入D-果糖8周诱导肝脂肪变性，随后同时给予益生菌和D-果糖8周。将42只6周龄雄性大鼠分配到不同的组，并补充不同的益生菌（500 mL饮用水中1 × 109 CFU）。8周后，采集血液和肝脏样本进行进一步评估，并检查与肝损伤对应的血浆和氧化应激标志物。 在八周内给予益生菌逆转了肝脏和血液甘油三酯浓度和血糖水平。此外，益生菌显着抑制肝脏组织中的氧化应激标志物。 虽然一些单一的益生菌制剂能够减轻氧化应激标志物，但益生菌的混合物显着改善了NAFLD动物的更多症状。这种增强的效果可能是由于益生菌的累积潜力，以维持氧化应激和提供改善的脂质分布，肝功能标志物和炎症标志物。
Treating nonalcoholic fatty liver disease (NAFLD) is considered one of the public health priorities in the past decade. So far, probiotics have represented promising results in controlling the signs and symptoms of NAFLD. However, attempts to find the ideal probiotic strain are still ongoing. The present study is designed to find the best strain amongst suitable probiotic strains according to their ability to ameliorate histopathological and oxidative stress biomarkers in hepatic steatosis-induced rats. Initially, four probiotics species, including Lactobacillus (L.) acidophilus, L. casei, L. reuteri, and Bacillus coagulans, were cultured and prepared as a lyophilized powder for animals. The experiment lasted for fifty days. Initially, hepatic steatosis was induced by excessive ingestion of D-fructose in rats for eight weeks, followed by eight weeks of administering probiotics and D-fructose concurrently. Forty-two six-week-old male rats were alienated to different groups and were supplemented with different probiotics (1∗109 CFU in 500 mL drinking water). After eight weeks, blood and liver samples were taken for further evaluation, and plasma and oxidative stress markers corresponding to liver injuries were examined. Administration of probiotics over eight weeks reversed hepatic and blood triglyceride concentration and blood glucose levels. Also, probiotics significantly suppressed markers of oxidative stress in the liver tissue. Although some of the single probiotic formulations were able to mitigate oxidative stress markers, mixtures of probiotics significantly ameliorated more symptoms in the NAFLD animals. This enhanced effect might be due to probiotics' cumulative potential to maintain oxidative stress and deliver improved lipid profiles, liver function markers, and inflammatory markers.
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影响因子：
--
作者：
Echeverría F;Valenzuela R;Bustamante A;Álvarez D;Ortiz M;Soto-Alarcon SA;Muñoz P;Corbari A;Videla LA
通讯作者：
Videla LA
影响因子：
2.4
作者：
Choi, Joo-Hee;Pichiah, P. B. Tirupathi;Cha, Youn-Soo
通讯作者：
Cha, Youn-Soo
影响因子：
3
作者：
Heidari, Reza;Taheri, Vahid;Najibi, Asma
通讯作者：
Najibi, Asma
影响因子：
5.9
作者：
Do MH;Lee E;Oh MJ;Kim Y;Park HY
通讯作者：
Park HY
影响因子：
3.9
作者：
Boutagy NE;McMillan RP;Frisard MI;Hulver MW
通讯作者：
Hulver MW