Alteration of sensory-evoked metabolic and oscillatory activities in the olfactory bulb of GLAST-deficient mice.
Alteration of sensory-evoked metabolic and oscillatory activities in the olfactory bulb of GLAST-deficient mice.
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DOI：
10.3389/fncir.2012.00001
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发表时间：
2012
影响因子：
3.5
通讯作者：
Gurden H
中科院分区：
文献类型：
--
作者：
Martin C;Houitte D;Guillermier M;Petit F;Bonvento G;Gurden H
星形胶质细胞是三方突触和神经血管单位的关键细胞成分。为了完成突触活动和代谢的双重作用，它们表达一组感知谷氨酸的受体和转运体。其中，已知GLT-1和GLAST转运体调节兴奋性突触的细胞外谷氨酸浓度，从而调节谷氨酸受体信号传导。这些主要的摄取系统也参与神经元的能量供应。然而，GLAST在同时调节代谢和神经元活动中的功能作用目前尚不清楚。我们利用主嗅球具有吸引力的结构和功能特征来探索谷氨酸last对感觉信息处理的影响，同时分别探测肾小球和更深细胞层的谷氨酸摄取和神经元活动。利用GLAST基因敲除小鼠的气味诱发2-脱氧葡萄糖成像和局部场电位记录，我们在体内发现GLAST基因的缺失改变了嗅球网络中的葡萄糖摄取和神经元振荡。
Astrocytes are key cellular elements in both the tripartite synapse and the neurovascular unit. To fulfill this dual role in synaptic activity and metabolism, they express a panel of receptors and transporters that sense glutamate. Among them, the GLT-1 and GLAST transporters are known to regulate extracellular glutamate concentrations at excitatory synapses and consequently modulate glutamate receptor signaling. These major uptake systems are also involved in energy supply to neurons. However, the functional role of GLAST in concurrent regulation of metabolic and neuronal activity is currently unknown. We took advantage of the attractive structural and functional features of the main olfactory bulb to explore the impact of GLAST on sensory information processing while probing both glutamate uptake and neuronal activity in glomeruli and deeper cellular layers, respectively. Using odor-evoked 2-deoxyglucose imaging and local field potential recordings in GLAST knockout mice, we show in vivo that deletion of GLAST alters both glucose uptake and neuronal oscillations in olfactory bulb networks.
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